Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.

CD81 / TAPA-1 (1.3.3.22), CF568 conjugate, 0.1mg/mL

Imprimer

Référence BNC680391-100

Conditionnement : 100uL

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Marque : Biotium

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Téléphone : +1 850 650 7790

CD81 / TAPA-1 Monoclonal Mouse Antibody (1.3.3.22)

Product Attributes

Antibody number	#0391
Antibody reactivity (target)	CD81, TAPA-1
Antibody type	Primary
Host species	Mouse
Clonality	Monoclonal
Clone	1.3.3.22
Isotype	IgG1, kappa
Molecular weight	26 kDa
Synonyms	CD81; Target of the antiproliferative antibody 1 (TAPA-1); Tetraspanin-28; Tspan-28
Human gene symbol	CD81
Entrez gene ID	975
SwissProt	P60033
Unigene	54457
Immunogen	B-Cell line derived from a Burkitt lymphoma
Antibody target cellular localization	Exosomes/EVs, Plasma membrane
Verified antibody applications	Exosome staining (verified), Flow (surface) (verified), IF (verified), IHC (FFPE) (verified)
Species reactivity	Human, Rat
Expected antibody applications	Functional studies (published for clone), WB (published for clone)
Antibody application notes	Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunofluorescence: 0.5-1 ug/mL, Immunohistology (formalin): 0.5-1 ug/mL, For functional studies, order Ab without azide, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Western blotting 0.5-1 ug/mL, Optimal dilution for a specific application should be determined by user
Positive control	Ramos, Daudi, Raji, U266, HUT-78, HUT-102, Jurkat, HL-60, KG1a, A293, A431, MG63, MCF-7, and human lymphocytes. Tonsil or liver tissue.
Shipping condition	Room temperature
Storage Conditions	Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C
Shelf life	Guaranteed for at least 24 months from date of receipt when stored as recommended
Regulatory status	For research use only (RUO)
Antibody/conjugate formulation	Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Antibody research areas	Exosomes/EVs, Immunology
Product origin	Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.

Technology

CF® Dyes

Considerations for Immunofluorescence Staining

Extracellular Vesicle Research

Primary & Secondary Antibody Conjugates

Tech Tips

Tech Tip: Fluorescent Detection of EVs by Flow Cytometry

The Full Spectrum Blog

Blog: Bringing Light to Extracellular Vesicles

Highlighted Citations

Exosome profiling with CF® dye tetraspanin panel shows single tetraspanin immunocapture methods may limit cancer biomarker detection

High-throughput fluorescence correlation spectroscopy analysis of extracellular vesicle markers

Product Description

This antibody recognizes a protein of 26 kDa, identified as CD81 (Workshop VI; Code CD81.1). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspanin transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.

Also view our CD81 Recombinant Monoclonal Mouse Biotium Choice Antibody (2304.81). This antibody belongs to the Biotium Choice list of select antibodies that have been developed and validated in-house for optimal performance. The antibody has been validated in flow cytometry and immunofluorescence microscopy.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email info@clinisciences.com to inquire about stock status and lead times before placing your order.

Catalog number key for antibody number 0391, Anti-CD81|TAPA-1 (1.3.3.22)

Antibody # prefix	Conjugation	Ex/Em (nm)	Laser line	Detection channel	Dye Features
BNC04	CF®405S	404/431	405	DAPI (microscopy), AF405	CF®405S Features
BNC88	CF®488A	490/515	488	GFP, FITC	CF®488A Features
BNC68	CF®568	562/583	532, 561	RFP, TRITC	CF®568 Features
BNC94	CF®594	593/614	561	Texas Red®	CF®594 Features
BNC40	CF®640R	642/662	633-640	Cy®5	CF®640R Features
BNC47	CF®647	650/665	633-640	Cy®5	CF®647 Features
BNC74	CF®740	742/767	633-685	775/50	CF®740 Features
BNCB	Biotin	N/A	N/A	N/A
BNUB	Purified	N/A	N/A	N/A
BNUM	Purified, BSA-free	N/A	N/A	N/A

Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.

Note: Listed references are for this antibody clone sold by Biotium and other suppliers.