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Flow Cytometry
Application

Flow Cytometry

Cells from BALB/c mesenteric lymph nodes were double-stained with Cd8a monoclonal antibody, clone 53-6.7 (FITC) (Cat # MAB6105) and Cd4 monoclonal antibody, clone GK1.5-PE, gated on small lymphocytes and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).

  • Specifications

    Product Description

    Rat monoclonal antibody raised against native Cd8a.

    Immunogen

    Native purified from mouse thymus/spleen cells.

    Host

    Rat

    Reactivity

    Mouse

    Specificity

    Specificity alpha chain of the murine CD8 heterodimer, Mr 32-34 KDa

    Form

    Liquid

    Conjugation

    APC/Cy5.5

    Isotype

    IgG2a, kappa

    Recommend Usage

    Flow Cytometry (0.2 ug/106 cells)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (0.09% sodium azide)

    Storage Instruction

    Store in the dark at 4°C. Do not freeze.
    Avoid prolonged exposure to light.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Immunohistochemistry (Frozen sections)

    Flow Cytometry

    Cells from BALB/c mesenteric lymph nodes were double-stained with Cd8a monoclonal antibody, clone 53-6.7 (FITC) (Cat # MAB6105) and Cd4 monoclonal antibody, clone GK1.5-PE, gated on small lymphocytes and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
  • Gene Info — Cd8a

    Entrez GeneID

    12525

    Gene Name

    Cd8a

    Gene Alias

    BB154331, Ly-2, Ly-35, Ly-B, Lyt-2

    Gene Description

    CD8 antigen, alpha chain

    Gene Ontology

    Hyperlink

    Gene Summary

    O

    Other Designations

    Lyt-2.1 lymphocyte differentiation antigen (AA at 100)

  • Publication Reference