- More Files
- Specifications
Product Description
Rat monoclonal antibody raised against native Cd8a.
Immunogen
Native purified from mouse thymus/spleen cells.
Host
Rat
Reactivity
Mouse
Specificity
Specificity alpha chain of the murine CD8 heterodimer, Mr 32-34 KDa
Form
Liquid
Conjugation
APC/Cy5.5
Isotype
IgG2a, kappa
Recommend Usage
Flow Cytometry (0.2 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Immunohistochemistry (Frozen sections)
Flow Cytometry
Cells from BALB/c mesenteric lymph nodes were double-stained with Cd8a monoclonal antibody, clone 53-6.7 (FITC) (Cat # MAB6105) and Cd4 monoclonal antibody, clone GK1.5-PE, gated on small lymphocytes and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). - Gene Info — Cd8a
- Publication Reference
- Extrathymic differentiation of intraepithelial lymphocytes: generation of a separate and unequal T-cell repertoire?
Lefrancois L.
Immunology Today 1991 Dec; 12(12):436.
- Phenotypic complexity of intraepithelial lymphocytes of the small intestine.
Lefrancois L.
Journal of Immunology 1991 Sep; 147(6):1746.
- The Lyt-2, Lyt-3 macromolecules: structural and functional studies.
Ledbetter JA, Seaman WE.
Immunological Reviews 1982 Jan; 68:197.
- Extrathymic differentiation of intraepithelial lymphocytes: generation of a separate and unequal T-cell repertoire?