CD9 Mouse Monoclonal Antibody (HI9a), CF®647Plus Conjugate - Biotium Choice

Note: CD9 clone HI9a is being discontinued and has been replaced by CD9 Recombinant Monoclonal Mouse Antibody (2310.9) – Biotium Choice, which has been validated to have the same bright and specific staining of human CD9.

CD9 (clone HI9a) is a mouse monoclonal antibody that recognizes the human tetraspanin protein CD9. This antibody belongs to the Biotium Choice list of select antibodies that have been validated and optimized in-house for optimal performance. This antibody has been validated in flow cytometry, in immunofluorescence microscopy, and in western blot. The antibody is available conjugated to R-PE or Astral™ tandem dyes. They are supplied in PBS, 0.1% BSA, 0.05% azide.

CD9 is a type IV transmembrane glycoprotein with four transmembrane domains. CD9 on pre-B cells may play a role in cell-cell adhesion. In addition, CD9 may play a role in signal transduction mediated by interaction with low molecular weight GTP binding proteins. CD9 is expressed on early B cells, eosinophils, basophils and activated T cells and is a major component of the platelet cell surface. It is also expressed on most non-T acute lymphoblastic leukemia cells and on some acute myeloid and chronic lymphoid leukemia.

Tetraspanin proteins CD9, CD63, and CD81, are the most common proteins used as exosome markers. Tetraspanins are plasma membrane proteins with many proposed functions, including activation and sorting of other membrane proteins. They are also thought to play a role in the targeting of proteins to multivesicular bodies (MVBs) and exosomes. These tetraspanins are broadly expressed on many cell types and can therefore be detected on many types of exosomes, but their expression levels vary depending on the cell type of origin.

As an isotype control, we recommend Isotype Control, Monoclonal Mouse IgG1 Kappa (IGG1/1331) for this antibody. View our full catalog of Biotium Choice antibodies, optimized and validated for the best results in flow cytometry.

Exosome Stains and Antibodies

Biotium also offers ExoBrite™ CD9 Flow Antibody and ExoBrite™ CD9 Western Antibody that are validated for optimal detection of isolated exosomes by flow cytometry or western blotting. Learn more about our products for exosome research.

CD9 Monoclonal Mouse Antibody (HI9a) - Biotium Choice