CF®594 Rabbit Anti-Human IgG (H+L), Highly Cross-Adsorbed

Référence 20072-1mg

Conditionnement : 1mg

Marque : Biotium

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Rabbit Anti-Human IgG (H+L), Highly Cross-Adsorbed

Highly cross-adsorbed rabbit anti-human IgG (H+L) secondary antibody labeled with our superior CF® dyes.

Technology

CF® Dyes
Considerations for Immunofluorescence Staining
Secondary Antibody Product Listings

Tech Tips

Protocol: Fluorescent Western Blotting
Protocol: Immunofluorescence Staining of Cells for Microscopy
Tech Tip: Combined Direct and Indirect IF Using Primary Antibodies from the Same Host

Product Description

This is a highly cross-adsorbed rabbit anti-human IgG (H L) secondary antibody labeled with our bright and photostable CF® dyes. To minimize cross-reactivity, the antibody has been adsorbed against mouse serum.

  • Highly cross-adsorbed for specific staining with minimal background
  • Available in 3 bright and photostable CF® dyes
  • Suitable for western, immunofluorescence, and immunohistology in FFPE tissues

Rabbit Anti-Human IgG (H+L), Highly Cross-Adsorbed

Conjugation Ex/Em Size Catalog No.
CF®488A 490/515 nm 50 uL (100 ug) 20071-1
0.5 mL (1 mg) 20071
1 mg 20071-1mg
CF®594 593/614 nm 50 uL (100 ug) 20072-1
0.5 mL (1 mg) 20072
1 mg 20072-1mg
CF®633 630/650 nm 50 uL (100 ug) 20066-1
0.5 mL (1 mg) 20066
1 mg 20066-1mg

View our full selection of Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.

CF® Dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.

Storage and Handling

Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.

Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:

CF® Dye and biotin conjugates: add 0.5 mL dH2O

HRP or DNP conjugates: add 1 mL dH2O

Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.

Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.

Note: Storage of the antibody for more than a day at final working dilution is not recommended.

CF is a registered trademark of Biotium, Inc.

References

Download a list of CF® dye references.