K1023-1 | CFDA SE Cell Tracer Kit Clinisciences

Background

The CFDA SE (carboxyfluorescein diacetate succinimidyl ester) Cell Tracer Kit is usually used in fluorescence analyses as a long-term tracer of cells. The Kit can be used for in vitro and in vivo labeling of cells to determine whether or not a cell is proliferating, especially.

CFDA SE is a cell-permeable dye, which could bind covalently to all free amines on the surface and inside of cells. It shows little cytotoxicity, with minimal observed effect on the proliferative ability or biology of cells. The intense fluorescent staining provided by CFDA SE which distinguish labeled cells from auto-fluorescence can enable the visualization even after several days post stain, making it an ideal stain for cell tracing studies.

Quality Control

Protocol

Components and Storage

Material	Amount	Storage	Stability
CFDA SE	10 vials, 1 mg each, lyophilized powder	≤-20°C Desiccate Protect from light and moisture Avoid repeated freezing and thawing	When stored as directed, kit will be stable for at least 6 months.
DMSO	1 vial of 2 mL
Approximate fluorescence excitation/emission maxima: 492/517 nm, after hydrolysis.

Specialty

Superior performance—the kit allows for bright, uniform staining of cells
Long-term retention—dye is well-retained in cells even after several days post stain
Non-toxic—minimal cytotoxicity for in vivo and in vitro experiment
Simple and convenient staining method

Chemical Name

5-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl diacetate

Cas No.

150347-59-4

Formula

C₂₉H₁₉NO₁₁

Solubility

Soluble in DMSO

Canonical SMILES

O=C1N(OC(C2=CC=C(C3(C(C=CC(OC(C)=O)=C4)=C4OC5=C3C=CC(OC(C)=O)=C5)OC6=O)C6=C2)=O)C(CC1)=O

Storage

Store at ≤-20°C Desiccate. Protect from light and moisture. Avoid repeated freezing and thawing.

CFDA SE Cell Tracer Kit

Référence K1023-1

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