Colo-824 growing culture
Référence 330463
Conditionnement : 1cellcultureflask
Marque : CLS Cell Lines Service
Colo-824 Cells
General information
Description | The cells do not tolerate DMSO. Upon thawing, DMSO has to be removed immediately by centrifugation. |
---|---|
Organism | Human |
Tissue | Breast, mammary gland |
Disease | Squamous cell carcinoma |
Metastatic site | Pleural effusion |
Synonyms | COLO-824, COLO 824, Colo 824, COLO #824, COLO824, Colo824, Colorado 824 |
Characteristics
Age | 52 years |
---|---|
Gender | Female |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Adherent/suspension |
Identifiers / Biosafety / Citation
Citation | Colo-824 (Cytion catalog number 300463) |
---|---|
Biosafety level | 1 |
Expression / Mutation
Protein expression | p53 negative |
---|---|
Tumorigenic | Yes, in nude mice |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
---|---|
Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 3 days |
Subculturing | Gather the suspension cells in a 15 ml tube and gently wash the adherent cells with PBS lacking calcium and magnesium (use 3-5 ml for T25 flasks and 5-10 ml for T75 flasks). Apply Accutase (1-2 ml for T25 flasks, 2.5 ml for T75 flasks) ensuring full coverage of the cell layer. Allow the cells to incubate at room temperature for 10 minutes. Following incubation, combine and centrifuge both the suspension and adherent cells. After centrifugation, carefully resuspend the cell pellet and transfer the cell suspension into new flasks containing fresh medium. |
Split ratio | A ratio of 1:4 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
|
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
---|---|
STR profile | Amelogenin: x,x CSF1PO: 10,12 D13S317: 12,13 D16S539: 11,12,13 D5S818: 12 D7S820: 8,11 TH01: 7,9 TPOX: 6,11 vWA: 16 D3S1358: 16,17 D21S11: 28 D18S51: 15,19 Penta E: 7 Penta D: 5,10 D8S1179: 12,14 FGA: 22 |
HLA alleles | A*: 01:01:01, 34:02:01 B*: 13:02:01, 44:03:01 C*: 04:01:01, 06:02:01 DRB1*: 07:01:01, 15:03:01G DQA1*: 01:02:01, 02:01:01 DQB1*: 02:02:01, 06:02:01 DPB1*: 04:01:01, 105:01:01 E: 01:01:01 |