Creatinine Assay Kit can be used to detect Creatinine in serum, plasma and urine using a microplate reader. Creatinine (Cr) can be catalyzed by creatinase, generating creatine. Creatine can be hydrolyzed into sarcosine and urea by creatinase. The sarcosine can be catalyzed by sarcosine oxidase to form glycine, formaldehyde and hydrogen peroxide. The reaction between hydrogen peroxide, 2,4-(6-Tri-iodine-3-hydroxybenzoic acid) and 4-ampyrone can be catalyzed by peroxidase, forming a pink compound. The Creatinine concentration can be calculated by measuring the absorbance at 515 nm.
Research Articles on Creatinine
| Target | Creatinine |
| Reactivity | General (All species) |
| Storage | Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual. |
| Validity | The validity for this kit is 12 months. |
| Test Range | 20.45 µmol/L – 800 µmol/L |
| Sensitivity | 3.8 µmol/L |
| Detection Method | Colorimetric |
| Assay Data | Quantitative |
| Sample Type | Serum, plasma and urine. |
| CAS Number | 60-27-5 |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |