Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.
 
> Cyclophilin A antibody [C1C3]

Cyclophilin A antibody [C1C3]

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Identifiez-vous

Référence GTX104698-100ul

Conditionnement : 100ul

Marque : Genetex

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Téléphone : +1 850 650 7790

Anti-Cyclophilin A antibody [C1C3] used in Western Blot (WB). GTX104698
Anti-Cyclophilin A antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX104698
Anti-Cyclophilin A antibody [C1C3] used in Western Blot (WB). GTX104698
Anti-Cyclophilin A antibody [C1C3] used in Western Blot (WB). GTX104698
Anti-Cyclophilin A antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104698
Anti-Cyclophilin A antibody [C1C3] used in Western Blot (WB). GTX104698
Anti-Cyclophilin A antibody [C1C3] used in Western Blot (WB). GTX104698
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Hamster
Package
100 μl,
25 μl
Datasheet File
View product citations for antibody GTX104698 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

Raji , HepG2 , mouse brain , 293T

Predict Reactivity

Rabbit, Sheep, Cat, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Cyclophilin A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240674

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peptidylprolyl isomerase A , CYPA , CYPH , HEL-S-69p

Background

This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported. [provided by RefSeq]

Database

DATA IMAGES

Anti-Cyclophilin A antibody [C1C3] used in Western Blot (WB). GTX104698

GTX104698 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cyclophilin A antibody [C1C3] (GTX104698) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclophilin A antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX104698

GTX104698 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft, using Cyclophilin A(GTX104698) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cyclophilin A antibody [C1C3] used in Western Blot (WB). GTX104698

GTX104698 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2
12% SDS PAGE
GTX104698 diluted at 1:3000
Anti-Cyclophilin A antibody [C1C3] used in Western Blot (WB). GTX104698

GTX104698 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cyclophilin A antibody [C1C3] (GTX104698) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclophilin A antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104698

GTX104698 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Cyclophilin A(GTX104698) antibody at 1:200 dilution.

Anti-Cyclophilin A antibody [C1C3] used in Western Blot (WB). GTX104698

GTX104698 WB Image

Cyclophilin A antibody detects Cyclophilin A protein by Western blot analysis. Mouse tissue extracts (50 μg) was separated by 15 % SDS-PAGE, and the membrane was blotted with Cyclophilin A antibody (GTX104698) at a dilution of 1:20000.

Anti-Cyclophilin A antibody [C1C3] used in Western Blot (WB). GTX104698

GTX104698 WB Image

Cyclophilin A antibody detects Cyclophilin A protein by western blot analysis. Raji whole cell extracts and cytoplasma+membrane and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclophilin A antibody (GTX104698) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Application Reference

V Belli et al. Genes 2023; 14 (9) : 1766 New Insights into Dyskerin-CypA Interaction: Implications for X-Linked Dyskeratosis Congenita and Beyond
Application : WB, ICC/IF
Conte M et al. Int J Mol Sci 2021; 23 (1) Relevance of AIF/CypA Lethal Pathway in SH-SY5Y Cells Treated with Staurosporine.
Application : WB
Reactivity : Human
Shih HJ et al. Sci Rep 2021; 11 (1) : 22925 Differential expression of hypoxia-inducible factors related to the invasiveness of epithelial ovarian cancer.
Application : WB
Reactivity : Human
Lin C.Y. et al. Natural Product Communications 2021; (Epub) Tanshinone IIA Shows Higher Antiproliferative Activities than Sinapic Acid in 4 Cancer Cell Lines and Simultaneously Induces Apoptosis and Necroptosis in Human Lung Cancer A549 Cells.
Application : WB
Reactivity : Human
Shih HJ et al. Am J Cancer Res 2020; 10 (6) : 1728-1744 IGFBP3 inhibits angiogenesis through intracellular regulation of THBS1 expression.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

Cyclophilin A antibody [C1C3] Cat. No. GTX104698

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-Cyclophilin A antibody [C1C3] used in Western Blot (WB). GTX104698
Date : Anonymous submitted on 24-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : OC3 cells
Amount used : 80 μg
Blocking : 5 % milk, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Application Reference
V Belli et al. Genes 2023; 14 (9):1766 New Insights into Dyskerin-CypA Interaction: Implications for X-Linked Dyskeratosis Congenita and Beyond
Application : WB, ICC/IF
Conte M et al. Int J Mol Sci 2021; 23 (1) Relevance of AIF/CypA Lethal Pathway in SH-SY5Y Cells Treated with Staurosporine.
Application : WB
Reactivity : Human
Shih HJ et al. Sci Rep 2021; 11 (1):22925 Differential expression of hypoxia-inducible factors related to the invasiveness of epithelial ovarian cancer.
Application : WB
Reactivity : Human
Lin C.Y. et al. Natural Product Communications 2021; (Epub) Tanshinone IIA Shows Higher Antiproliferative Activities than Sinapic Acid in 4 Cancer Cell Lines and Simultaneously Induces Apoptosis and Necroptosis in Human Lung Cancer A549 Cells.
Application : WB
Reactivity : Human
Shih HJ et al. Am J Cancer Res 2020; 10 (6):1728-1744 IGFBP3 inhibits angiogenesis through intracellular regulation of THBS1 expression.
Application : WB
Reactivity : Human
Chen JF et al. J Pharm Biomed Anal 2020; 187:113142 Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells.
Application : WB
Reactivity : Human
Rosales Ramirez R et al. J Virol 2019; 93 (4) The Dengue Virus Nonstructural Protein 1 (NS1) Is Secreted from Mosquito Cells in Association with the Intracellular Cholesterol Transporter Chaperone Caveolin Complex.
Romel Rosales Ramirez et al. bioRxiv 2018; (Epub) The dengue virus non-structural protein 1 (NS1) is secreted from mosquito cells in association with the intracellular cholesterol transporter chaperone caveolin complex.
Application : ICC/IF
Reactivity : Hamster
Lin CY et al. Cell Death Discov 2016; 2:16065 Simultaneous induction of apoptosis and necroptosis by Tanshinone IIA in human hepatocellular carcinoma HepG2 cells.
Application : WB
Reactivity : Human
May EW et al. Pharmacol Res 2014; 90C:43117 identification of up- and down-regulated proteins in *-resistant uterine cancer cells: reticulocalbin-1 plays a key role in the development of *-associated resistance.
Application : WB
Reactivity : Human
Huang HJ et al. Mol Biosyst 2014; (Epub) Proteomic analysis of rhein-induced cyt: ER stress mediates cell death in breast cancer cells.
Application : WB
Reactivity : Human
Fan CY et al. Electrophoresis 2013; 34 (24):3305-14 Proteomic and redox-proteomic study on the role of glutathione reductase in human lung cancer cells.
Application : WB
Reactivity : Human
SDS
Glycerol.pdf
Thimerosal.pdf

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