CYPIVF8 (CYP4F8) (NM_007253) Human 3' UTR Clone

CAT#: SC203597

3' UTR clone of cytochrome P450 family 4 subfamily F polypeptide 8 (CYP4F8) for miRNA target validation


Product Images

Specifications

Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Symbol CYPIVF8
Synonyms CPF8; CYPIVF8
ACCN NM_007253
Insert Size 1311 bp
Sequence Data
>SC203597 3’UTR clone of NM_007253
The sequence shown below is from the reference sequence of NM_007253. The complete sequence of this clone may contain minor differences, such as SNPs.
Blue=Stop Codon Red=Cloning site

GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
CTTTGGCTGCGAGTAGAACCCCTGGGCTGAGGCCTGCAGTGACCCACCCACCTACCTTTGCATCACCTA
CCTTTGCACCAACTACCTTTTCAGATTTCCGGTAATAAATCTGTGTTGGCCCCTGTGCCTCAGTCCCGC
GGATGGCCAGTAGGGGGCGCTGGAGGACTGCGGGGATCTAGGGCCTGGCTGGGAAGAGGCGGGGAGATG
TCTCTGTGCCCAAGATACTCACTGCCTCTCTGGGTGAGCACAGGAGCCCCGTGCTGAGGGTGGGATCTC
CCAGAGTCTAAGTAAAGACTTTTTCCCCCCCAAAATAATTGTGTATTCTGATATAAATTTTTGCCAATT
TAGAATCCCTGTTTTTTAGCTAGGTGCATAGCAGCCTGAAATACAGATCACATTTGAAAGCCTTTCTTG
AAGCTCAATTGGTTAAGTGACTGTGGCTGTCCCATGTGTAGAAGCCAAAGATTATGTGGTAATTCTGGT
AACCTTTCTTAAGAGAAAGCTGCTACAGTCTGTCTGCTCAGTCCTCCCCTCTTCCTTCCTGCTGCTTGG
AAGATTTTTTTTTTTTTTTGAGACATGGTGTCTCTCGTCGCCCAGGCCGGAGTGCAGTGGCTCACTGCA
ACCTCCACCTCCTGGGTTCAAGTGATTCTCCTGACTCAGTCGCCTGAGTAGATAGGATAACAGGCGCCT
ACCACCACACCCGGCTAATTGTATTTTTAGTACAGATGGGGTTTTGCTATGTTGGCCAGGCTGGTCTCA
AACTCCTGGCCTCAAGTGATCAGCCCACCTCAGCCTCCCAAAGTGCCAGAATTGCAGGTGTGAGAATAA
AAATTCTTGAGTTTCAATGTTTATTTTAGAATCAGGTGCTAACATGCAAATTTATTACAAAGGTATATT
GCATCATGCTGAGGTTTGGAGTATGAATGACTCCATCACCCATGTAGTGAGCATAGTATCTAATAGGCA
GTTTTTCAGCTTTTCCCCCTCCCTGCTTCCCCTGTCTAGTAGTCCCCAGGATCTGTTGTTCCCATCTTT
ATTACCATGTGTATCCAATGCATACCTCCCACTTATAAGTGAGAACATGCAATATTTTGTTTTCTGTTC
CTGCATTAGTTTGCTTAGGATAATGGCCCCCAGCTGCATCTATGTTGCTGCAAACGACATGATTTTGTA
CTTTTTAATGACTGCATAGTATTTCATGGTGTATTTCTACACATTTTCTTTATCCACTCCACCATTGAT
GGGCACCTGTGTTGATTCAGCATCTTCGCTATTGTGATTAGAGCTGCGATAAACATGTGGATGCTTGTG
ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
Restriction Sites SgfI-MluI     
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_007253.4
Summary This gene, CYP4F8, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and functions as a 19-hydroxylase of prostaglandins in seminal vesicles. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. Another member of this family, CYP4F3, is approximately 18 kb away. [provided by RefSeq, Jul 2008]
Locus ID 11283
MW 49.2

Documents