Cytokeratin 8/18(C-51), 0.2mg/mL
Référence BNUB0034-500
Conditionnement : 500uL
Marque : Biotium
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Téléphone : +1 850 650 7790
| Antibody number | #0034 |
|---|---|
| Antibody reactivity (target) | Cytokeratin 8/18 |
| Antibody type | Primary |
| Host species | Mouse |
| Clonality | Monoclonal |
| Clone | C-51 |
| Isotype | IgG1, kappa |
| Molecular weight | 52.5 kDa (CK8); 45 kDa (CK18) |
| Synonyms | CARD2; CK8; CYK8; CYKER; Cytokeratin Endo A; DreK8; EndoA; K2C8; K8; Keratin 8; Krt 2.8; KRT8; Type-II Keratin Kb8 |
| Human gene symbol | KRT8 & KRT18 |
| Entrez gene ID | 3856 (CK8); 3875 (CK18) |
| SwissProt | P05787 (CK8); P05783 (CK18) |
| Unigene | 533782 & 708445 (CK8); 406013 (CK18) |
| Immunogen | Cytoskeleton preparation from HeLa cells |
| Antibody target cellular localization | Cytoskeleton |
| Verified antibody applications | IHC (FFPE) (verified) |
| Species reactivity | Cow, Human, Monkey, Pig, Sheep |
| Expected antibody applications | IF (published for clone), WB (published for clone) |
| Antibody application notes | Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunofluorescence: 1-2 ug/mL, Does not react with mouse, rat, ferret, rabbit, donkey, chicken, Xenopus; others not known, Immunohistology formalin-fixed 0.5-1.0 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Western blotting 0.5-1.0 ug/mL, Optimal dilution for a specific application should be determined by user |
| Positive control | MCF-7 or A431 cells. Skin, Colon, lung or breast carcinoma |
| Shipping condition | Room temperature |
| Storage Conditions | Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C |
| Shelf life | Guaranteed for at least 24 months from date of receipt when stored as recommended |
| Regulatory status | For research use only (RUO) |
| Antibody/conjugate formulation | Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide |
| Antibody research areas | Cancer, Cytoskeleton |
| Product origin | Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot. |
| Cell/tissue expression | Epithelial cells |
| Tumor expression | Breast cancer, Hepatocellular carcinoma, Paget cell marker, Sarcoma |
Product Description
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.
Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the
CF® Dye Brochure
for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.info@clinisciences.com
to inquire about stock status and lead times before placing your order.Catalog number key for antibody number 0034, Anti-Cytokeratin 8 (C-51)
| Antibody # prefix | Conjugation | Ex/Em (nm) | Laser line | Detection channel | Dye Features |
|---|---|---|---|---|---|
| BNC04 | CF®405S | 404/431 | 405 | DAPI (microscopy), AF405 | CF®405S Features |
| BNC88 | CF®488A | 490/515 | 488 | GFP, FITC | CF®488A Features |
| BNC68 | CF®568 | 562/583 | 532, 561 | RFP, TRITC | CF®568 Features |
| BNC94 | CF®594 | 593/614 | 561 | Texas Red® | CF®594 Features |
| BNC40 | CF®640R | 642/662 | 633-640 | Cy®5 | CF®640R Features |
| BNC47 | CF®647 | 650/665 | 633-640 | Cy®5 | CF®647 Features |
| BNC74 | CF®740 | 742/767 | 633-685 | 775/50 | CF®740 Features |
| BNCB | Biotin | N/A | N/A | N/A | |
| BNUB | Purified | N/A | N/A | N/A | |
| BNUM | Purified, BSA-free | N/A | N/A | N/A |
References
Note: References for this clone sold by other suppliers may be listed for expected applications.
- Brain Pathol (1999) 9: 617-626. (IHC, FFPE)
- Br J Cancer (1999) 81(5): 769-773. (WB)
- Int J Cancer (2004) 111: 662-668. (WB)
- Cell (2014) 159(1): 163-175. (IF; IHC, FFPE)