C3362-5mg | DAPI (hydrochloride) [28718-90-3] Clinisciences

DAPI (hydrochloride) is a fluorescent probe which serves as a DNA-specific probe for flow cytometry, chromosome staining, as well as DNA visualization and quantitation in histochemistry and biochemistry. DAPI forms a fluorescent complex by attaching to the minor grove of consecutive (3 ~ 4 base pairs) A-T rich sequences of DNA. Although DAPI is capable of binding to other sequences of double stranded DNA, or double stranded RNA, these complexes are much less fluorescent. DAPI can be used alone for cell cycle studies of drug effects, or in combination with another fluorochrome for multi-parameter cell analysis. One of the most popular stains combines DAPI with sulforhodamine (SR 101) for simultaneous measurement of DNA and protein content in cells. DAPI can be used on either fixed or live cells, but its low permeability in live cells suggests that higher concentrations should be used.

References:

1. Kapuscinski J. DAPI: a DNA-specific fluorescent probe. Biotechnic & Histochemistry, 1995, 70(5): 220-233.

2. Zink D, Sadoni N, Stelzer E. Visualizing chromatin and chromosomes in living cells. Methods, 2003, 29(1): 42-50.

1. Mei Liu, et al. "Shear Bond Strength to Enamel, Mechanical Properties and Cellular Studies of Fiber-Reinforced Composites Modified by Depositing SiO2 Nanofilms on Quartz Fibers via Atomic Layer Deposition." Int J Nanomedicine. 2024 Mar 5:19:2113-2136. PMID: 38476282
2. ZHOU Qiyue, HONG Gaoying, et al. "Characterization and biocompatibility analysis of different silanes coupling c (RGDfK) cyclic peptide on titanium surfaces." J Prev Treat Stomatol Dis, 2022, 30(6): 398⁃405.
3. Liang Y, Wang J. "Sorafenib-Induced Exosome Secretion Promotes Che-motherapy Resistance in Hepatoma Cell Line." Japanese J Gstro Hepato. 2021; V6(12): 1-7.

Storage	Store at -20°C
M.Wt	350.25
Cas No.	28718-90-3
Formula	C₁₆H₁₇Cl₂N₅
Solubility	insoluble in EtOH; ≥10 mg/mL in H2O; ≥53.3 mg/mL in DMSO
Chemical Name	2-(4-carbamimidoylphenyl)-1H-indole-6-carboximidamide dihydrochloride
SDF	Download SDF
Canonical SMILES	NC(C(C=C1)=CC=C1C2=CC3=CC=C(C(N)=N)C=C3N2)=N.Cl.Cl
Shipping Condition	Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips	We do not recommend long-term storage for the solution, please use it up soon.

Purity = 98.00%

DAPI (hydrochloride) [28718-90-3]

Référence C3362-5mg

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