Product Description  

Discover-sc Single Cell WGA Kit is based on multiple displacement amplification (MDA) and is  designed for unbiased whole genome amplification from single cell and other micro samples.  The sizes of amplification products are between 2 kb to 100 kb, with a coverage greater than  95% and an average length higher than 15 kb. The products can be widely applied to whole  genome sequencing, whole exome sequencing, large fragment copy number variation analysis,  microsatellite analysis, qPCR analysis, or gene chip analysis. The Phi29 DNA polymerase used in this kit is cloned from phage and has a strong strand  displacement activity. Melting and replication of the DNA chain with complex structure can be  achieved. Meanwhile, Phi29 has a strong chain affinity and can achieve up to 100 kb of continuous polymerization, and the amplification product can be applied to almost all of the downstream  genome analysis. Phi29 DNA polymerase also possesses a potent 3'→5 'exonuclease (proofreading)  activity, its fidelity is 1,000-fold that of Taq enzyme, thus ensuring the high fidelity of DNA replication. All the reagents provided in the kit have undergone rigorous quality control and functional testing  to ensure the optimal stability and repeatability.

 Features  

• Wide sample compatibility: suitable for animal and plant cells, bacteria or blastomeres, trophectoderm cells, sperm and other samples.

• High coverage: after single-cell genome amplification, the coverage of more than 95% can be achieved.

• High uniformity: can be used for >1 Mb chromosome copy number variation analysis.

• High fidelity: The fidelity of Phi29 DNA polymerase is 1000 times higher than that of Taq enzyme.

• Simple operation: single-tube reaction, the operation time is less than 10 minutes, and the amplification product does not need to be purified.

 Components  

 Storage  

Store at -30 ~ -15℃ and transport at ≤0℃. For long-term storage, please store at -85 ~ -65℃.