Orb1291288-96 | Dog PDGFBB ELISA Kit Clinisciences

Dog PDGFBB ELISA Kit

Catalog Number	orb1291288
Category	Assays and Kits
Description	Dog PDGFBB (Platelet Derived Growth Factor BB) ELISA Kit
Tested applications	ELISA
Reactivity	Canine
Range	31.25-2000 pg/mL
Concentration	2000 pg/mL
Solubility (25°C)	2000 pg/mL
Sensitivity	12.8 pg/mL
Alternative names	PDGF-BB
Note	For research use only
Application notes	Standard: 2000 pg/mL. Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog PDGFBB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog PDGFBB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog PDGFBB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog PDGFBB in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Expiration Date	Please enquire.