Application
| WB, IHC-P, IF, E |
|---|---|
| Primary Accession | Q9Y2E6 |
| Other Accession | NP_055992, 148237498 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | Predicted: 68 kDa Observed: 68 kDa |
| Application Notes | DTX4 antibody can be used for detection of DTX4 by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 23220 |
|---|---|
| Target/Specificity | DTX4; DTX4 antibody is human and mouse reactive. At least two isoforms of DTX4 are known to exist; this antibody will detect both isoforms. DTX4 antibody is predicted to not cross-react with other DTX protein family members. |
| Reconstitution & Storage | DTX4 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | DTX4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | DTX4 |
|---|---|
| Synonyms | KIAA0937, RNF155 |
| Function | Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations (By similarity). Functions as a ubiquitin ligase protein in vivo, mediating 'Lys48'-linked polyubiquitination and promoting degradation of TBK1, targeting to TBK1 requires interaction with NLRP4. |
| Cellular Location | Cytoplasm. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The Deltex 4 E3 ubiquitin ligase (DTX4) is a member of a protein family that is involved in Notch signaling and neurogenesis (1). Recent studies have shown that DTX4 is also associated with the innate immune response (2). Following the activation of type I interferon signaling, the Nod-like receptor protein 4 (NLRP4) recruits DTX4 for Lys48-linked polyubiquitination and degradation of the kinase TBK1 (NAK), thereby maintaining immune homeostasis during antiviral innate immunity (2).
References
Kishi N, Tang Z, Maeda Y, et al. Murine homologs of deltex define a novel gene family involved in vertebrate Notch signaling and neurogenesis. Int. J. Dev. Neurosci. 2001; 19:21-35.
Cui J, Li Y, Zhu L, et al. NLRP4 negatively regulated type I interferon signaling by targeting the kinase TBK1 for degradation via the ubiquitin ligase DTX4. Nat. Immunol. 2012; 13:387-95.