EasyPure® Blood RNA Kit
Référence ER401-01
Conditionnement : 50rxns
Marque : TransGen Biotech
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Téléphone : +1 850 650 7790
ManualEasyPure® Blood RNA Kit provides a simple and fast column based method to isolate total RNA from 50 μl-1.5 ml of fresh or anticoagulated blood. Blood is lysed and DNA is digested with DNase I. RNA is bound to silica membrane. After washing, high quality RNA is eluted. Purified RNA is suitable for RT-PCR, qRT-PCR and Northern blot.
Storage
DNase I and DNase I Reaction Buffer at -20°C for one year; others at room temperature (15-25°C) in a dry place for one year.
Shipping
DNase I and DNase I Reaction Buffer on dry ice (-70 °C); others at room temperature.
| Component | ER401-01 (50 rxns) |
| Red Cell Lysis Buffer 2 (RCL2) | 125 ml |
| Binding Buffer 7 (BB7) | 40 ml |
| Clean Buffer 7 (CB7) | 60 ml |
| Wash Buffer 7 (WB7) | 12 ml |
| DNase I (3 units/μl) | 1500 units |
| DNase I Reaction Buffer | 4×1 ml |
| RNase-free Water | 10 ml |
| RNA Spin Columns with Collection Tubes | 50 each |
| RNase-free Tube (1.5 ml) | 50 each |
Shuhong C , Jing X , Feng L , et al. Correlation of Serum Soluble Interleukin-7 Receptor and Anti-C1q Antibody in Patients with Systemic Lupus Erythematosus[J]. Autoimmune Diseases, 2016, 2016:1-11.