Fast Mutagenesis System
Référence FM111-01
Conditionnement : 10rxns
Marque : TransGen Biotech
Contactez votre distributeur local :
Téléphone : +1 850 650 7790
Manual• Primers anneal to the DNA template, mutant strands are synthesized with 2×TransStart® FastPfu PCR SuperMix.
• In vitro digestion of non-mutated parental plasmid (methylated plasmid) with DMT enzyme and in vivo degradation of non-mutated parental plasmid (methylated plasmid) with DMT Chemically Competent Cell, so as to efficiently select mutant clones.
• Mutation sites on both primers to improve mutation efficiency.
• Partially overlapping primers for exponential DNA amplification.
• Fast (4 kb/min) and high fidelity ( 54-fold fidelity as compared to EasyTaq® DNA Polymerase) 2xTransStart® FastPfu PCR SuperMix for DNA amplification.
• Double digestions (in vitro and in vivo) of parental plasmids to enhance mutation efficiency.
Storage
Competent Cell at -70°C for six months; others at -20°C for two years
Shipping
Dry ice (-70℃)
| Component | FM111-01 (10 rxns) | FM111-02 (20 rxns) |
| 2×TransStart® FastPfu Fly PCR SuperMix | 250 μl | 500 μl |
| DMT Enzyme (10 units/μl) | 10 μl | 20 μl |
| DMT Chemically Competent Cell | 10×50 μl | 20×50 μl |
| Nuclease-free Water | 1 ml | 1 ml |
| SControl Plasmid (5 ng/μl) | 10 μl | 20 μl |
| SControl Primers (10 μM ) | 10 μl | 20 μl |
Wang J, Zhou L, Shi H, et al. A single transcription factor promotes both yield and immunity in rice[J].Science, 2018, 7, 361(6406):1026-1028.
Li W , Zhu Z , Chern M , et al. A Natural Allele of a Transcription Factor in Rice Confers Broad-Spectrum Blast Resistance[J]. Cell, 2017, 170(1):114-126.e15.
Zhou X, Wang B, Pan Q, et al. Whole-genome sequencing of the snub-nosed monkey provides insights into folivory and evolutionary history[J]. Nature Genetics, 2014, 46(12):1303-1310.
Zhang H , Lin X , Han Z , et al. Crystal structure of PXY-TDIF complex reveals a conserved recognition mechanism among CLE peptide-receptor pairs[J]. Cell Research, 2016, 26(5):543-55.
Song N , Liu Z S , Xue W , et al. NLRP3 Phosphorylation Is an Essential Priming Event for Inflammasome Activation[J]. Molecular Cell, 2017, 68(1):185-197.