Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.
 
> Fast Red ITR [97-35-8]

Fast Red ITR [97-35-8]

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Référence HY-W040295-1ml

Conditionnement : 10mM/1mL

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Fast Red ITR is a nonlinear optical material. Fast Red ITR can be used in alkaline phosphatase histochemistry.

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Fast Red ITR Chemical Structure

Fast Red ITR Chemical Structure

CAS No. : 97-35-8

This product is a controlled substance and not for sale in your territory.

Based on 1 publication(s) in Google Scholar

Description

Fast Red ITR is a nonlinear optical material[1]. Fast Red ITR can be used in alkaline phosphatase histochemistry[1][2].

Masse moléculaire

258.34

Formule

C11H18N2O3S
CAS No.

97-35-8
Appearance

Solid

Color

White to off-white

SMILES

CCN(S(=O)(C1=CC(N)=C(C=C1)OC)=O)CC
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvant et solubilité
In Vitro: 

DMSO : 100 mg/mL (387.09 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.8709 mL 19.3543 mL 38.7087 mL
5 mM 0.7742 mL 3.8709 mL 7.7417 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Calculateur de molarité

  • Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (9.68 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (9.68 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation

Purity: 98.87%

COA (250 KB) HNMR (244 KB) LCMS (98 KB)

Instruction de manipulation (2659 KB)
Références

  • [1]. Shinzo Muto, et al. Second-and third-harmonic generation of Nd: glass laser in fast red ITR organic crystal fiber. Japanese Journal of Applied Physics. 1998.

    [2]. Ziomek CA, et al. A highly fluorescent simultaneous azo dye technique for demonstration of nonspecific alkaline phosphatase activity. J Histochem Cytochem. 1990 Mar;38(3):437-42.  [Content Brief]

  • [1]. Shinzo Muto, et al. Second-and third-harmonic generation of Nd: glass laser in fast red ITR organic crystal fiber. Japanese Journal of Applied Physics. 1998.

    [2]. Ziomek CA, et al. A highly fluorescent simultaneous azo dye technique for demonstration of nonspecific alkaline phosphatase activity. J Histochem Cytochem. 1990 Mar;38(3):437-42.

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.8709 mL 19.3543 mL 38.7087 mL 96.7717 mL
5 mM 0.7742 mL 3.8709 mL 7.7417 mL 19.3543 mL
10 mM 0.3871 mL 1.9354 mL 3.8709 mL 9.6772 mL
15 mM 0.2581 mL 1.2903 mL 2.5806 mL 6.4514 mL
20 mM 0.1935 mL 0.9677 mL 1.9354 mL 4.8386 mL
25 mM 0.1548 mL 0.7742 mL 1.5483 mL 3.8709 mL
30 mM 0.1290 mL 0.6451 mL 1.2903 mL 3.2257 mL
40 mM 0.0968 mL 0.4839 mL 0.9677 mL 2.4193 mL
50 mM 0.0774 mL 0.3871 mL 0.7742 mL 1.9354 mL
60 mM 0.0645 mL 0.3226 mL 0.6451 mL 1.6129 mL
80 mM 0.0484 mL 0.2419 mL 0.4839 mL 1.2096 mL
100 mM 0.0387 mL 0.1935 mL 0.3871 mL 0.9677 mL
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Keywords:

Fast Red ITR97-35-8Othersnonlinearoptical materialInhibitorinhibitorinhibit