Sample Type:|Whole blood, serum, plasma, erythrocytes, urine, cell lysates, and tissue samples||Target:|Glutathione and Oxidized Glutathione||Method of Analysis:|Absorbance Plate Reader||Intended Use:|Quantitatively measure glutathione (GSH) and oxidized glutathione (GSSG) present in a variety of samples, including whole blood, serum, plasma, erythrocytes, urine, cell lysates, and tissue samples. Analyze the absorbance colorimetric signal by spectrophotometer.||Test Principle:|Glutathione, gamma-L-glutamyl-L-cysteinyl-glycine or GSH, is the most abundant non-protein thiol in cells. GSH is involved in the regulation of a number of essential biochemical processes within the cell. Primarily recognized as a key intracellular source of reducing power for combating the toxic accumulation of free radical byproducts, GSH is also involved with detoxifying and removal of exo/endogenous toxins and alkylating agents. In its role as a cell signaling agent, GSH is involved in DNA synthesis and cell proliferation regulation. Due to the genetically conserved molecular processes by which cells die, intracellular levels of GSH can favor a cell death pathway via apoptosis (adequate intracellular GSH stores) or via necrosis or autophagy (depleted intracellular GSH stores). Detection of a drop in intracellular GSH concentration in an experimental cell population relative to a negative (healthy cell) control is often indicative of an apoptosis induction event. Glutathione Colorimetric Detection BioAssay™ Kit is designed to quantitatively measure GSH and GSSG in a variety of samples.||Kit Components:|4 clear half-area polystyrene 96-well microtiter plates|Oxidized Glutathione (GSSG) Standard, 1x250uM, 1x350uL|Colorimetric GSH Detection Substrate, 1x1mL|Glutathione Assay Buffer, 1x225mL|NADPH Concentrate, 1x1mL|Glutathione Reductase Concentrate, 1x1mL||Storage and Stability:|Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.