Glycogenin 1 (GYG1) (NM_001184721) Human Tagged ORF Clone
CAT#: RC229819
- TrueORF®
GYG1 (Myc-DDK-tagged)-Human glycogenin 1 (GYG1), transcript variant 3
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
Product Images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | Glycogenin 1 |
| Synonyms | GSD15; GYG |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | >RC229819 representing NM_001184721 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGATCAGGCCTTTGTGACACTAACCACAAACGATGCCTACGCCAAAGGTGCCCTGGTCCTGGGAT CATCTCTGAAACAGCACAGGACCACCAGGAGGCTGGTCGTGCTCGCCACCCCTCAGGTCTCAGACTCCAT GAGAAAAGTTTTAGAGACAGTCTTTGATGAAGTCATCATGGTAGATGTCTTGGACAGTGGCGATTCTGCT CATCTAACCTTAATGAAGAGGCCAGAGTTGGGTGTCACGCTGACAAAGCTCCACTGCTGGTCGCTTACAC AGTATTCAAAATGTGTATTCATGGATGCAGATACTCTGGTCCTAGCAAATATTGATGATCTTTTTGACAG AGAAGAATTGTCAGCAGCACCAGACCCAGGGTGGCCTGACTGCTTCAATTCCGGAGTCTTCGTTTATCAG CCTTCAGTTGAAACATACAATCAGCTGTTGCATCTTGCTTCTGAGCAAGGTAGTTTTGATGGTGGGGACC AAGGCATACTGAACACATTTTTTAGCAGCTGGGCAACAACAGATATCAGAAAACACCTGCCGTTTATTTA TAACCTAAGCAGCATCTCTATATACTCCTACCTCCCGGCATTTAAAGTGAAGATGTCTCAGGAGCCATAT CACATCTGTCCCTTGGGGAGATCCCAGCTATGGCACAGCCGTTTGTATCCTCGGAAGAACGGAAGGAACG ATGGGAACAGGGCCAGGCTGATTATATGGGAGCAGATTCCTTTGACAACATCAAGAGGAAACTTGACACT TACCTCCAGTAGAAACACTGCATTTTTCTGTGAACACATCCACTTCACAAGCCTTGTTTCTGATACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC229819 representing NM_001184721 Red=Cloning site Green=Tags(s) MTDQAFVTLTTNDAYAKGALVLGSSLKQHRTTRRLVVLATPQVSDSMRKVLETVFDEVIMVDVLDSGDSA HLTLMKRPELGVTLTKLHCWSLTQYSKCVFMDADTLVLANIDDLFDREELSAAPDPGWPDCFNSGVFVYQ PSVETYNQLLHLASEQGSFDGGDQGILNTFFSSWATTDIRKHLPFIYNLSSISIYSYLPAFKVKMSQEPY HICPLGRSQLWHSRLYPRKNGRNDGNRARLIIWEQIPLTTSRGNLTLTSSRNTAFFCEHIHFTSLVSDT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms | CHROMATOGRAMS Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
| ACCN | NM_001184721 |
| ORF Size | 837 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001184721.1, NP_001171650.1 |
| RefSeq ORF | 840 bp |
| Locus ID | 2992 |
| UniProt ID | P46976 |
| Cytogenetics | 3q24 |
| MW | 31.9 kDa |
| Gene Summary | This gene encodes a member of the glycogenin family. Glycogenin is a glycosyltransferase that catalyzes the formation of a short glucose polymer from uridine diphosphate glucose in an autoglucosylation reaction. This reaction is followed by elongation and branching of the polymer, catalyzed by glycogen synthase and branching enzyme, to form glycogen. This gene is expressed in muscle and other tissues. Mutations in this gene result in glycogen storage disease XV. This gene has pseudogenes on chromosomes 1, 8 and 13 respectively. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Sep 2010] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| RC229819L3 | Lenti ORF clone of Human glycogenin 1 (GYG1), transcript variant 3, Myc-DDK-tagged | 600,00 EUR | |
| RC229819L4 | Lenti ORF clone of Human glycogenin 1 (GYG1), transcript variant 3, mGFP tagged | 600,00 EUR | |
| RG229819 | GYG1 (tGFP-tagged) - Human glycogenin 1 (GYG1), transcript variant 3 | 500,00 EUR | |
| SC328457 | GYG1 (untagged)-Human glycogenin 1 (GYG1) transcript variant 3 | 330,00 EUR |