GnRH (Gonadotropin Releasing Hormone) BioAssay™ ELISA Kit (Sheep)

Sample Type:|Serum, plasma, tissue homogenates and other biological fluids.||Intended Use:|Competitive ELISA, Coated with Antigen||Sensitivity:|<18.75pg/ml||Range:|31.25-2000pg/ml||Specificity:|Sheep||Test Principle:|This BioAssay™ ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with GnRH. During the reaction, GnRH in the sample or standard competes with a fixed amount of GnRH on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to GnRH. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of GnRH in the samples is then determined by comparing the O.D. of the samples to the standard curve.||Kit Components:|Microtiter Strips, 1x48 wells or 1x96 wells |Standard, 1x1 vial or 2x1 vials|Sample/Standard Dilution Buffer, 1x10ml or 1x20ml|Antibody (Biotin) (Concentrated), 1x30ul or 1x60ul|Antibody Dilution Buffer, 1x5ml or 1x10ml|Streptavidin (HRP) (SABC), 1x60ul or 1x120ul|SABC Dilution Buffer, 1x5ml or 1x10ml|TMB Substrate, 1x5ml or 1x10ml|Stop Solution, 1x5ml or 1x10ml|Wash Buffer, 25X, 1x15ml or 1x30ml||Storage and Stability:|Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.