Goat anti-ENPP1 / PC1
Référence EB06948
Conditionnement : 100ug
Marque : Everest Biotech
Code | Name | Applications | Availability | Size | Tested species | Grade |
---|---|---|---|---|---|---|
EB06948 | Goat Anti-ENPP1 / PC1 Antibody | Pep-ELISA, IHC | 100µg specific antibody in 200µl | Human |
Target Protein
Principal Names: ENPP1, PC-1, ectonucleotide pyrophosphatase/phosphodiesterase 1, HGNC:3356, M6S1, NPP1, NPPS, PCA1, PDNP1, Ly-41 antigen, OTTHUMP00000043194, alkaline phosphodiesterase 1, membrane component, chromosome 6, surface marker 1, phosphodiesterase I/nucleotide pyrophosphatase 1, plasma-cell membrane glycoprotein 1Official Symbol: ENPP1
Accession Number(s): NP_006199.2
Human GeneID(s): 5167
Immunogen
Peptide with sequence C-KTHLPTFSQED, from the C Terminus of the protein sequence according to NP_006199.2.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:64000.Western blot: Preliminary testing showed bands at approx., 25+150kDa in Human Liver lysates after 0.5-1ug/ml antibody staining (calculated Mwt of 105kDa according to NP_006199.2). Both bands were successfully blocked by incubation with the immunizing peptide, and the 150Kdaa band is observed by other sources. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Thyroid Gland. Recommended concentration: 4µg/ml.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Mouse, Rat
Specific References
This antibody (previous batch) has been successfully used in IHC on Mouse:
Takahito Mae, Tomoka Hasegawa, Hiromi Hongo, Tomomaya Yamamoto, Shen Zhao, Minqi, Yutaka Yamazaki, Norio Amizuka
Immunolocalization of Enzymes/Membrane Transporters Related to Bone Mineralization in the Metaphyses of the Long Bones of Parathyroid-Hormone-Administered Mice
Medicina (Kaunas). 2023 Jun 20;59(6):1179.
PMID: 37374382
This antibody (previous batch) has been successfully used in ICC on Mouse:
Tomomaya Yamamoto et al.
Comparative immunolocalization of tissue nonspecific alkaline phosphatase and ectonucleotide pyrophosphatase/phosphodiesterase 1 in murine bone
Journal of Oral Biosciences (2021), https://doi.org/10.1016/j.job.2021.08.001
PMID: 34391947
This antibody (previous batch) has been successfully used in Western Blot and IHC:
Lau WM, Doucet M, Stadel R, Huang D, Weber KL, Kominsky SL
Enpp1: a potential facilitator of breast cancer bone metastasis.
PLoS One. 2013 Jul 5;8(7):e66752.
PMID: 23861746
This antibody (previous batch) has been successfully used in WB:
Simão AM, Yadav MC, Narisawa S, Bolean M, Pizauro JM, Hoylaerts MF, Ciancaglini P, Millán JL.
Proteoliposomes harboring alkaline phosphatase and nucleotide pyrophosphatase as matrix vesicle biomimetics.
J Biol Chem. 2010 Mar 5;285(10):7598-609.
PMID: 20048161
- Western blot Guidelines
- Everest Western blot labeling
- Western blot Trouble shooting guide
- IHC staining on paraffin sections
- Immunofluorescence Protocol
- Flow Cytometry Protocol
- MSDS - All Goat antibodies
- Tissue Lysate Preparation
- Cell Lysate Preparation
- Blocking with the immunizing peptide
- LsBio IHC Protocol