Goat Anti-Mouse IgG(H+L), Rat ads-AP

Référence 1034-04

Conditionnement : 1.0mL

Marque : Southern Biotech

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Isotype Goat IgG
Specificity Reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, IgG2c, and IgG3 and with the light chains of mouse IgM and IgA
Source Pooled antisera from goats hyperimmunized with mouse IgG
Cross Adsorption Rat immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification Method Affinity chromatography on mouse IgG covalently linked to agarose
Conjugate AP (Alkaline Phosphatase)
Buffer Formulation 50 mM Tris/1 mM MgCl2/50% Glycerol containing < 0.1% sodium azide, pH 8.0
Clonality Polyclonal
Concentration Lot specific
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA 1-5
FLISA
Flow Cytometry 6-9

Other referenced applications for relevant formats include -
ELISpot 10
Western Blot 11-17
Immunohistochemistry-Frozen Sections 18
Immunocytochemistry 19
RRID Number AB_2794339
Documentation
Technical Bulletin Safety Datasheet

Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view

  1. 1. Shichkin VP, Spivak NY. Cytokine-deficient mice as a model for generation of autologous anti-cytokine monoclonal antibodies. Immunol Lett. 2006;102:148-57. (ELISA)
  2. 2. van Olffen RW. Modulating T cell homeostasis via TNF and TNFR superfamily members: characterization and function of effector & regulatory T cells [dissertation]. Amsterdam (Netherlands): University of Amsterdam, 2009. (ELISA)
  3. 3. Shichkin VP. Immune response of IL-4-knockout mice to low-dose immunization with autologous IL-4. WebmedCentral IMMUNOLOGY. 2011;2(12):WMC002826. (ELISA)
  4. 4. Suzuki K, Tsunoda H, Omiya R, Matoba K, Baba T, Suzuki S, et al. Structure of the plexin ectodomain bound by semaphorin-mimicking antibodies. PLoS One. 2016;11(6):e0156719. (ELISA)
  5. 5. Benyacoub J, Blum-Sperisen S, Rochat F, von der Weid T, inventors; Nestec S.A., assignee. Probiotics for use in expecting female mammals for enhancing the immunity of their offspring. United States patent application publication US 2017/0006908 A1. 2017 Jan 12. (ELISA)
  6. 6. Corral L, Takizawa H, Hanke T, Jamieson AM, Raulet DH. A new monoclonal antibody reactive with several Ly49 NK cell receptors mediates redirected lysis of target cells. Hybridoma. 1999;18:359-66. (FC)
  7. 7. Krasinskas AM, Eiref SD, McLean AD, Kreisel D, Gelman AE, Popma SH, et al. Replacement of graft-resident donor-type antigen presenting cells alters the tempo and pathogenesis of murine cardiac allograft rejection. Transplantation. 2000;70:514-21. (FC)
  8. 8. Kreisel D, Petrowsky H, Krasinskas AM, Krupnick AS, Szeto WY, McLean AD, et al. The role of passenger leukocyte genotype in rejection and acceptance of rat liver allografts. Transplantation. 2002;73:1501-7. (FC)
  9. 9. Arefanian H, Tredget EB, Rajotte RV, Korbutt GS, Gill RG, Rayat GR. Combination of anti-CD4 with anti-LFA-1 and anti-CD154 monoclonal antibodies promotes long-term survival and function of neonatal porcine islet xenografts in spontaneously diabetic NOD mice. Cell Transplant. 2007;16:787-98. (FC)
  10. 10. Huang C, Hatzi K, Melnick A. Lineage-specific functions of Bcl-6 in immunity and inflammation are mediated by distinct biochemical mechanisms. Nat Immunol. 2013;14:380-8. (ELISPOT)
  11. 11. Gill MB, Perez-Polo JR. Bax shuttling after rotenone treatment of neuronal primary cultures: effects on cell death phenotypes. J Neurosci Res. 2009;87:2047-65. (WB)
  12. 12. Perez-Polo JR, Reilly CB, Rea HC. Oxygen resuscitation after hypoxia ischemia stimulates prostaglandin pathway in rat cortex. Int J Dev Neurosci. 2011;29:639-44. (WB)
  13. 13. Durham-Lee JC, Wu Y, Mokkapati VU, Paulucci-Holthauzen AA, Nesic O. Induction of angiopoietin-2 after spinal cord injury. Neuroscience. 2012;202:454-64. (WB)
  14. 14. Bronner DN, O'Riordan MX, He Y. Caspase-2 mediates a Brucella abortus RB51-induced hybrid cell death having features of apoptosis and pyroptosis. Front Cell Infect Microbiol. 2013;3:83. (WB)
  15. 15. Guptarak J, Wanchoo S, Durham-Lee J, Wu Y, Zivadinovic D, Paulucci-Holthauzen A, et al. Inhibition of IL-6 signaling: A novel therapeutic approach to treating spinal cord injury pain. Pain. 2013;154:1115-28. (WB)
  16. 16. Xu X, Araki K, Li S, Han J, Ye L, Tan WG, et al. Autophagy is essential for effector CD8+ T cell survival and memory formation. Nat Immunol. 2014;15:1152-61. (WB)
  17. 17. Staffler G, Landlinger C, Le Bras M, inventors; AFFiRiS AG, assignee. Vaccines and monoclonal antibodies targeting truncated variants of osteopontin and uses thereof. United States patent application publication US 2017/0137509 A1. 2017 May 18. (WB)
  18. 18. Kawasaki T, Nishio T, Kawaguchi S, Kurosawa H. Spatiotemporal distribution of GAP-43 in the developing rat spinal cord: a histological and quantitative immunofluorescence study. Neurosci Res. 2001;39:347-58. (IHC-FS)
  19. 19. Zeineldin R, Rosenberg M, Ortega D, Buhr C, Chavez MG, Stack MS, et al. Mesenchymal transformation in epithelial ovarian tumor cells expressing epidermal growth factor receptor variant III. Mol Carcinog. 2006;45:851-60. (ICC)

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