GSK3145095 [1622849-43-7]
Référence HY-111946-1ml
Conditionnement : 10mM/1mL
Marque : MedChemExpress
GSK3145095 is a RIP1 kinase inhibitor with an IC50 of 6.3 nM .
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
GSK3145095 Chemical Structure
CAS No. : 1622849-43-7
This product is a controlled substance and not for sale in your territory.
Based on 1 publication(s) in Google Scholar
Voir tous les produits spécifiques à Isoform RIP kinase:
Description |
GSK3145095 is a RIP1 kinase inhibitor with an IC50 of 6.3 nM [1]. |
IC50 & Target |
IC50: 6.3 nM (RIP1 kinase )[1] |
||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
In Vitro |
GSK3145095 potently blocks the TNF response as shown by determination of overall cell viability as measured by cellular ATP levels (IC50 = 1.6 nM), cell death as measured by LDH release (IC50 = 0.5 nM) and RIP1-dependent inflammatory cytokine MIP-1β production, either as absolute levels for protein, or fold changes in mRNA expression (IC50 = 0.4 nM) [1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
||||||||||||
Essai clinique |
|
||||||||||||
Masse moléculaire |
397.38 |
||||||||||||
Formule |
C20H17F2N5O2 |
||||||||||||
CAS No. |
1622849-43-7 |
||||||||||||
Appearance |
Solid |
||||||||||||
Color |
White to off-white |
||||||||||||
SMILES |
FC1=CC(F)=CC2=C1NC([C@@H](NC(C3=NNC(CC4=CC=CC=C4)=N3)=O)CC2)=O |
||||||||||||
Livraison | Room temperature in continental US; may vary elsewhere. |
||||||||||||
Stockage |
|
||||||||||||
Solvant et solubilité |
In Vitro:
DMSO : 250 mg/mL (629.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
|
||||||||||||
Pureté et documentation |
Purity: 99.39% |
||||||||||||
Références |
|
Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
---|
DMSO | 1 mM | 2.5165 mL | 12.5824 mL | 25.1648 mL | 62.9121 mL |
5 mM | 0.5033 mL | 2.5165 mL | 5.0330 mL | 12.5824 mL | |
10 mM | 0.2516 mL | 1.2582 mL | 2.5165 mL | 6.2912 mL | |
15 mM | 0.1678 mL | 0.8388 mL | 1.6777 mL | 4.1941 mL | |
20 mM | 0.1258 mL | 0.6291 mL | 1.2582 mL | 3.1456 mL | |
25 mM | 0.1007 mL | 0.5033 mL | 1.0066 mL | 2.5165 mL | |
30 mM | 0.0839 mL | 0.4194 mL | 0.8388 mL | 2.0971 mL | |
40 mM | 0.0629 mL | 0.3146 mL | 0.6291 mL | 1.5728 mL | |
50 mM | 0.0503 mL | 0.2516 mL | 0.5033 mL | 1.2582 mL | |
60 mM | 0.0419 mL | 0.2097 mL | 0.4194 mL | 1.0485 mL | |
80 mM | 0.0315 mL | 0.1573 mL | 0.3146 mL | 0.7864 mL | |
100 mM | 0.0252 mL | 0.1258 mL | 0.2516 mL | 0.6291 mL |
GSK3145095 Related Classifications
- Apoptosis
- RIP kinase