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> Guinea Pig Polyclonal Anti-Keratin K8/K18

Guinea Pig Polyclonal Anti-Keratin K8/K18

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Conditionnement : 100ul

Marque : Progen

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anti-Keratin K8/K18 guinea pig polyclonal, serum

Datasheet
Key Features
  • Stabilized antiserum
  • Guinea pig polyclonal
  • Suitable for ELISA, IHC and WB
  • Reacts with bovine, chicken, human, mouse, rat and hamster
Product description
Quantity 100 µl
Antibody Type Polyclonal
Host Guinea pig
Conjugate Unconjugated
Application ELISA, IHC, WB
Purification Stabilized antiserum
Reactivity Bovine, Chicken, Hamster, Human, Mouse, Rat
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Keratin K8/K18 filaments, reconstituted from purified bovine keratins K8 and K18
Formulation Contains 0.09% sodium azide
UniprotID P05786 (Bovine), A0A1L1RIW5 (Chicken), P05787 (Human), P11679 (Mouse), Q10758 (Rat)
Synonym Keratin, type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8, KRT8, CYK8
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:100
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (microwave treatment recommended)
ELISA Assay dependent
Western Blot (WB) 1:1,500-1:2,500
Background

Reactive polypeptide (specificity): Mr 52,500 and 45,500; Keratins K8 and K18 (formerly also designated cytokeratins 8 and 18) of simple type epithelium.
Tumors specifically detected: all adenocarcinoma tested, undifferentiated carcinoma, hepatocellular carcinoma, cervix carcinoma.


References/Publications (33)
Publication
Species
Application
Publication Kim, M. et al. Plakophilin 1 deficiency in prostatic tumours is correlated with immune cell recruitment and controls the up-regulation of cytokine expression post-transcriptionally. FEBS J. (2022).
Species human
Application IHC-IF (paraffin)
Publication Kakehashi, A. et al. Canopy homolog 2 as a novel molecular target in hepatocarcinogenesis. Cancers (Basel). 13, 3613 (2021).
Species mouse
Application IHC
Publication Komai, M. et al. Pathological Study on Epithelial-Mesenchymal Transition in Silicotic Lung Lesions in Rat. Vet.Sci. 6, (2019)
Species rat
Application IHC (paraffin)
Publication Hollern, D. et al. A mouse model featuring tissue-specific deletion of p53 and Brca1 gives rise to mammary tumors with genomic and transcriptomic similarities to human basal-like breast cancer. Breast.Cancer.Res.Treat. 174, 143-155 (2019)
Species mouse
Application IHC-IF (paraffin)
Publication Williams, J. et al. Epithelial and stromal circadian clocks are inversely regulated by their mechano-matrix environment. J.Cell.Sci. 131,  (2018).
Species mouse
Application ICC-IF
Publication Sarrach, S. et al. Spatiotemporal patterning of EpCAM is important for murine embryonic endo- and mesodermal differentiation. Sci.Rep. 8, 1801 (2018).
Species mouse
Application IHC (frozen)
Publication Linde, N. et al. Macrophages orchestrate breast cancer early dissemination and metastasis. Nat.Commun. 9, 21 (2018).
Species mouse
Application IHC (frozen)
Publication Song, Y. et al. RB inactivation in keratin 18 positive thymic epithelial cells promotes non-cell autonomous T cell hyperproliferation in genetically engineered mice. PLoS One 12, e0171510 (2017)
Species mouse
Application ICC-IF
Publication Kakehashi, A. et al. Progression of Hepatic Adenoma to Carcinoma in Ogg1 Mutant Mice Induced by Phenobarbital. Oxid.Med.Cell.Longev. 2017, 8541064 (2017).
Species mouse
Application IHC (paraffin)
Publication Méndez-Gómez, H. R. et al. Transcytosis in the blood-cerebrospinal fluid barrier of the mouse brain with an engineered receptor/ligand system. Mol. Ther. Methods Clin. Dev. 2, (2015).
Species mouse
Application IHC (free floating sections)
Publication Russell, R. et al. Loss of ATM accelerates pancreatic cancer formation and epithelial-mesenchymal transition. Nat. Commun. 6, 1–16 (2015).
Species mouse
Application IHC
Publication Yang, C. et al. Plakophilin 1-deficient cells upregulate SPOCK1: implications for prostate cancer progression. Tumour Biol. 36, 9567–77 (2015).
Species human
Application IHC (paraffin)
Publication Dunphy, K. A. et al. Oncogenic transformation of mammary epithelial cells by transforming growth factor beta independent of mammary stem cell regulation. Cancer Cell Int. 13, (2013).
Species mouse
Application IHC (paraffin),ICC-IF
Publication Song, Y. et al. Carcinoma Initiation via Rb Tumor Suppressor Inactivation: A Versatile Approach to Epithelial Subtype- Dependent Cancer Initiation in Diverse Tissues. PLoS One 8, (2013).
Species mouse
Application IHC (paraffin)
Publication König, K. et al. Loss of the keratin cytoskeleton is not sufficient to induce epithelial mesenchymal transition in a novel KRAS driven sporadic lung cancer mouse model. PLoS One 8, e57996 (2013).
Species mouse
Application IHC (paraffin)
Publication Clemons, N. J. et al. Sox9 drives columnar differentiation of esophageal squamous epithelium: a possible role in the pathogenesis of Barrett’s esophagus. AJP Gastrointest. Liver Physiol. 303, G1335–G1346 (2012).
Species mouse
Application WB
Publication Kumar, P. et al. Cooperativity of Rb, Brca1, and p53 in Malignant Breast Cancer Evolution. PLoS Genet. 8, (2012).
Species mouse
Application IHC (paraffin)
Publication Simmons, M. J., Serra, R., Hermance, N. & Kelliher, M. A. NOTCH1 inhibition in vivo results in mammary tumor regression and reduced mammary tumorsphere-forming activity in vitro. Breast Cancer Res. 14, R126 (2012).
Species mouse
Application WB,IHC (paraffin),ICC-IF
Publication Bennecke, M. et al. Ink4a/Arf and Oncogene-Induced Senescence Prevent Tumor Progression during Alternative Colorectal Tumorigenesis. Cancer Cell 18, 135–146 (2010).
Species mouse
Application IHC (paraffin)
Publication Yan, H. et al. Pathways Contributing to Development of Spontaneous Mammary Tumors in BALB/c-Trp53+/− Mice. Am. J. Pathol. 176, 1421–1432 (2010).
Species mouse
Application IHC (paraffin)
Publication Straub, B. K. et al. Lipid droplet-associated PAT-proteins show frequent and differential expression in neoplastic steatogenesis. Mod. Pathol. 23, 480–492 (2010).
Species human
Application IHC (frozen)
Publication Eispert, A.-C. et al. Evidence for distinct populations of human Merkel cells. Histochem. Cell Biol. 132, 83–93 (2009).
Species human
Application IHC (frozen)
Publication Li, Y.-C., Bai, W.-Z., Sakai, K. & Hashikawa, T. Fluorescence and Electron Microscopic Localization of F-actin in the Ependymocytes. J. Histochem. Cytochem. Histochem Cytochem 57, 741–751 (2009).
Species rat
Application IHC (free floating sections)
Publication Straub, B. K., Stoeffel, P., Heid, H., Zimbelmann, R. & Schirmacher, P. Differential pattern of lipid droplet-associated proteins and de novo perilipin expression in hepatocyte steatogenesis. Hepatology 47, 1936–1946 (2008).
Species human
Application IHC (frozen)
Publication Bultman, S. J. et al. Characterization of mammary tumors from Brg1 heterozygous mice. Oncogene 27, 460–468 (2008).
Species mouse
Application IHC (paraffin)
Publication Hüsemann, Y. et al. Systemic Spread Is an Early Step in Breast Cancer. Cancer Cell 13, 58–68 (2008).
Species mouse
Application ICC-IF
Publication Herschkowitz, J. I. et al. Identification of conserved gene expression features between murine mammary carcinoma models and human breast tumors. Genome Biol. 8, (2007).
Species human
Application IHC (paraffin)
Publication Mikaelian, I. et al. Expression of Terminal Differentiation Proteins Defines Stages of Mouse Mammary Gland Development. Vet. Pathol. 43, 36–49 (2006).
Species mouse
Application WB,IHC (paraffin)
Publication Moll, I. et al. Human Merkel cells--aspects of cell biology, distribution and functions. Eur. J. Cell Biol. 84, 259–71 (2005).
Species human
Application IHC (frozen)
Publication Galiè, M. et al. Mammary carcinoma provides highly tumourigenic and invasive reactive stromal cells. Carcinogenesis 26, 1868–1878 (2005).
Species mouse
Application IHC (paraffin)
Publication Greten, F. R. et al. Stat3 and NF-kB Activation Prevents Apoptosis in Pancreatic Carcinogenesis. Gastroenterology 123, 2052–2063 (2002).
Species mouse
Application WB
Publication Knapp, A. C. & Franke, W. W. Spontaneous losses of control of cytokeratin gene expression in transformed, non-epithelial human cells occurring at different levels of regulation. Cell 59, 67–79 (1989).
Species human
Application WB,ICC-IF
Publication Franke, W. W. & Moll, R. Cytoskeletal components of lymphoid organs. I. Synthesis of cytokeratins 8 and 18 and desmin in subpopulations of extrafollicular reticulum cells of human lymph nodes, tonsils, and spleen. Differentiation. 36, 145–63 (1987).
Species human
Application IHC (frozen),IEM
Downloads
File
Category
Size
Filetype
File IHC guinea pig antibody
Category Protocol
Size 167.92 KB
Filetype pdf
File WB protocol
Category Protocol
Size 210.69 KB
Filetype pdf
Datasheet
MSDS
FAQs
  • PVDF membranes show better results than nitrocellulose (higher capacity, allows for more stringent washing conditions in case of background problems).
  • Use freshly prepared blocking solution (e.g. 5% nonfat dry milk, 0.05% Tween 20), block for at least 1 h at room temperature.
  • Use the antibody in a higher dilution, but prolong incubation time and exposure time.
  • Always use a fresh aliquot of the antibody.
  • Do not repeatedly freeze the antibody (eventually centrifuge shortly after thawing to remove cryo-precipitates).
  • Include an additional washing step.
    You might also try more stringent wash conditions, e.g. add 0.5 M NaCl to the wash buffer.
  • Always use a fresh aliquot of secondary antibody.
  • In case you use ECL most the guinea pig antibody should be diluted further in order to get rid of the background.

The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
  1. Methanol/ acetone fixation: Immerse slide in precooled (-20°C) methanol for 5 min, immerse in precooled (-20°C) acetone for 30-60 sec, let specimen air dry before antibody incubation.
  2. Methanol/ acetone fixation plus detergent permeabilization: After methanol/ acetone fixation and air-drying dip slide either in a solution containing 0.1-0.2% Triton X-100 in PBS or in 0.1% saponin in PBS for 1-5 min at room temperature (enhances accessibility of many cytoskeletal antigens).

  • Air-drying of the section.
  • Block with the serum of the species in which the secondary antibody was raised for 30 min.
  • Incubation with 1st antibody 1 h at RT in moist chamber.
  • Wash 3x with PBS.
  • Incubation with appropriate fluorescent secondary antibody, 30-60 min at RT.
  • Wash 3x with PBS.
  • Immerse shortly into ethanol.
  • Let air dry.
  • Cover with mounting medium.

Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.

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Keratin

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