HIST4H4 Mouse Monoclonal Antibody [Clone ID: C14691]
Specifications
Product Data | |
Clone Name | C14691 |
Applications | IF, IP, WB |
Recommended Dilution | Western blot: 1 μg/ml for chemiluminescence detection system. Immunoprecipitation: 5 μg/200 μl of cell extract from 5x106 cells. Immunocytochemistry: 5 μg/ml. |
Reactivities | Hamster, Human, Mouse, Rat |
Host | Mouse |
Isotype | IgG2b |
Clonality | Monoclonal |
Immunogen | Protein fraction extracted from the mitotic chromosome. |
Specificity | This antibody reacts with Histone H4. |
Formulation | PBS containing 50% Glycerol, pH 7.2. No preservative is contained. State: Azide Free State: Liquid purified Ig fraction |
Concentration | lot specific |
Purification | Protein A Agarose Chromatography |
Conjugation | Unconjugated |
Storage | Upon receipt, store (in aliqouts) at -20°C. Avoid repeated freezing and thawing. |
Stability | Shelf life: One year from despatch. |
Predicted Protein Size | 11 kDa |
Gene Name | histone cluster 4, H4 |
Database Link | |
Background | The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The N-terminal tail of core histones undergoes multiple different post-transcriptional modifications including acetylation, phosphorylation, ubiquitination, methylation, and ADP-ribosylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. Acetylation of histone H4 plays a primary role in the structural changes that mediate enhanced binding of transcription factors to their recognition sites within nucleosomes. It has been suggested that acetylated Histone H4 also is involved in heightened activation of the transcription of male X chromosomes, and that Histone H4 stimulates glucose transport activity in rat skeletal muscle. |
Synonyms | HIST1H4, H4/A, H4FA |
Note | This product was originally produced by MBL International. Protocol: SDS-PAGE & Western Blotting Immunoprecipitation Immunofluorescence microscopy |
Reference Data |
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