Histone H3 K9me3 antibody
Référence orb420398-50ug
Conditionnement : 50ug
Marque : Biorbyt
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Histone H3 K9me3 antibody
Catalog Number: orb420398
| Catalog Number | orb420398 |
|---|---|
| Category | Antibodies |
| Description | Histone H3 K9me3 antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ChIP, DOT, IF, IHC, WB |
| Reactivity | C. elegans, Human |
| Isotype | IgG |
| Immunogen | Histone H3 [Trimethyl Lys9] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic trimethylated peptide surrounding Lysine 9 of human Histone H3.2. |
| Concentration | 0.49 mg/ml |
| Dilution range | ChIP: 2-5µg/million cells, IHC: 1:1000, IF: 1:1000, WB: 1:5000 |
| Form/Appearance | Liquid (sterile filtered) |
| Purity | Anti-Histone H3 [Trimethyl Lys9] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined. |
| Conjugation | Unconjugated |
| UniProt ID | Q71DI3 |
| NCBI | NP_001005464 |
| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Alternative names | rabbit anti-Histone H3 trimethyl Lys9 antibody, H3 |
| Note | For research use only |
| Application notes | Anti-Histone H3 [Trimethyl Lys9] antibody is tested by Western Blot, Chromatin Immunoprecipitation, Dot Blot, Immunocytochemistry, and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus antibody production in collaboration with Novus Biologicals. |
| Expiration Date | 12 months from date of receipt. |
Chromatin Immunoprecipitation of Anti Histone H3 [Trimethyl Lys9] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 ug of Anti-Histone H3K9Me3 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input = 1).
Dot Blot of Rabbit Histone H3 [Trimethyl Lys9] Antibody. Lane 1: K9 KMe1. Lane 2: K9 KMe2. Lane 3: K9 KMe3. Lane 4: K9. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [Trimethyl Lys9] antibody at 1:1000 for 45 min at 4°C. Secondary antibody: Dylight™488 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.
Immunofluorescence of Rabbit Anti-Histone H3 [Trimethyl Lys9] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [Trimethyl Lys9] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10000 for 45 min at RT. Localization: Histone H3 [Trimethyl Lys9] is nuclear and chromosomal. Staining: Histone H3 [Trimethyl Lys9] is expressed in green and the nuclei are counterstained with DAPI (blue).
Western Blot of Rabbit Anti-Histone H3 [Trimethyl Lys9] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [Trimethyl Lys9] at 1:5000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.
Western Blot of Rabbit Anti-Histone H3 [Trimethyl Lys9] Antibody. Lane 1: HeLa histone preps. Load: 30 µg per lane. Primary antibody: Histone H3 [Trimethyl Lys9] at 1:5000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.