Hsp70 Antibody
Référence OASE00333
Conditionnement : 100ul
Marque : Aviva Systems Biology
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| Datasheets/Manuals | Printable datasheet for OASE00333 |
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| Predicted Species Reactivity | Human, Mouse, Rat, Beluga, Bovine, Dog, Fish, Carp, Guinea Pig, Hamster, Monkey, Pig, Sheep, Coral, Plant, Shark, L. amazonensis |
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| Product Format | Liquid PBS with 50% glycerol and 0.09% sodium azide |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugation | Unconjugated |
| Application | WB, IHC, ICC, IF, IP, ELISA |
| :: | Certificate of Analysis: A 1:10,000 dilution was sufficient for detection of hsp70 in 20ug of HeLa cell lysate by ECL immunoblot analysis. Cellular Localization: Cytoplasm |
| Reconstitution and Storage | Store at -20C. Shipping with Blue Ice or 4C. |
| Immunogen | Recombinant Full length HSP70 Protein |
| Purification | Peptide Affinity Purified |
| Concentration | 1 mg/ml |
| Specificity | Detects a ~70kDa. May cross-react with HSC70 at lower dilutions. |
| Dilution | WB (1:1000), IHC (1:100), ICC/IF (1:100), IP (1:100); optimal dilutions for assays should be determined by the user. |
| Application Info | WB: 1:1000 IHC: 1:100 ICC/IF: 1:100 IP: 1:100 |
| Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
| Reference | 1. Welch W.J. and Suhan J.P. (1986) J.Cell Biol. 103: 2035-2050. 2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J. Mol. Evol. 38(1): 1-17. 3. Rothman J. (1989) Cell 59: 591 -601. 4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887. 5. Bork P., Sander C. & Valencia A. (1992) Proc. Nut1 Acad. Sci. USA 89: 7290-7294. 6. Fink A.L. (1999) Physiol. Rev. 79: 425-449. 7. Hung T.H., et al. (2001) Am J Pathol. 159: 1031-1043. 8. Locke M. (2000) Cell Stress & Chaperones 5: 45-51. 9. Ianaro A., et al. (2001) FEBS Lett. 508: 61-66. 10.Trentin G.A. et al. (2001) J Biol Chem. 276: 13087- 13095. |
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| Gene Symbol | HSP70 |
| Gene Full Name | heat shock 70kDa protein 1A |
| Alias Symbols | HSP70 1, HSP70 2, HSP70.1, HSP72, HSP73, HSPA1, HSPA1A, HSPA1B |
| NCBI Gene Id | 3303 |
| Description of Target | HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity. The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides. When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half. The structure of this ATPbinding domain displays multiple features of nucleotide binding proteins. All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein. The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. |
| Uniprot ID | P0DMV8/P0DMV9 |
| Protein Accession # | NP_005336.3 |
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What is the species homology for "Hsp70 Antibody (OASE00333)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Beluga, Bovine, Dog, Fish, Carp, Guinea Pig, Hamster, Monkey, Pig, Sheep, Coral, Plant, Shark, L. amazonensis".
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How long will it take to receive "Hsp70 Antibody (OASE00333)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "Hsp70 Antibody (OASE00333)" provided in?
This item is provided in "Liquid PBS with 50% glycerol and 0.09% sodium azide".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Hsp70 Antibody (OASE00333)"?
This target may also be called "HSP70 1, HSP70 2, HSP70.1, HSP72, HSP73, HSPA1, HSPA1A, HSPA1B" in publications.
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What is the shipping cost for "Hsp70 Antibody (OASE00333)"?
The shipping cost for this item is within the US. Please contact us for specific shipping for international orders.
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What is the guarantee for "Hsp70 Antibody (OASE00333)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Hsp70 Antibody (OASE00333)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Hsp70 Antibody (OASE00333)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "HSP70"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "HSP70"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "HSP70"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "HSP70"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "HSP70"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "HSP70"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.