General Info
| Applications: | ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.049ng/mL |
| Detection Limit: | 0.156-10ng/mL |
| Short Description: | This CYP2D6 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 4.5 hrs |
Information
| Gene Symbol: | CYP2D6 |
| Gene ID: | 1565 |
| Uniprot ID: | CP2D6_HUMAN |
| Sample Type: | serum, plasma, tissue homogenates or other biological fluids. |
Description
| Tissue Specificity | |
| Post Translational Modifications | |
| Function | A cytochrome P450 monooxygenase involved in the metabolism of fatty acids, steroids and retinoids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the epoxidation of double bonds of polyunsaturated fatty acids (PUFA). Metabolizes endocannabinoid arachidonoylethanolamide (anandamide) to 20-hydroxyeicosatetraenoic acid ethanolamide (20-HETE-EA) and 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acid ethanolamides (EpETrE-EAs), potentially modulating endocannabinoid system signaling. Catalyzes the hydroxylation of carbon-hydrogen bonds. Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis. Catalyzes the oxidative transformations of all-trans retinol to all-trans retinal, a precursor for the active form all-trans-retinoic acid. Also involved in the oxidative metabolism of drugs such as antiarrhythmics, adrenoceptor antagonists, and tricyclic antidepressants. |
| Protein Name | Cytochrome P450 2d6Cypiid6Cholesterol 25-HydroxylaseCytochrome P450-Db1Debrisoquine 4-Hydroxylase |
| Database Links | Reactome: R-HSA-211935Reactome: R-HSA-211958Reactome: R-HSA-211981Reactome: R-HSA-211999Reactome: R-HSA-9027307Reactome: R-HSA-9749641 |
| Cellular Localisation | Endoplasmic Reticulum MembranePeripheral Membrane ProteinMicrosome Membrane |
| Alternative ELISA Names | Cytochrome P450 2d6 ELISA kitCypiid6 ELISA kitCholesterol 25-Hydroxylase ELISA kitCytochrome P450-Db1 ELISA kitDebrisoquine 4-Hydroxylase ELISA kitCYP2D6 ELISA kitCYP2DL1 ELISA kit |
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Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance