General Info
| Applications: | ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.138ng/mL |
| Detection Limit: | 0.312-20ng/mL |
| Short Description: | This HDAC9 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 4.5 hrs |
Information
| Gene Symbol: | HDAC9 |
| Gene ID: | 9734 |
| Uniprot ID: | HDAC9_HUMAN |
| Sample Type: | tissue homogenates, cell lysates or other biological fluids. |
Description
| Tissue Specificity | Broadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level). |
| Post Translational Modifications | Phosphorylated on Ser-220 and Ser-450.which promotes 14-3-3-binding, impairs interaction with MEF2, and antagonizes antimyogenic activity. Phosphorylated on Ser-240.which impairs nuclear accumulation. Isoform 7 is phosphorylated on Tyr-1010. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import. Sumoylated. |
| Function | Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription. Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter. |
| Protein Name | Histone Deacetylase 9Hd9Histone Deacetylase 7bHd7Hd7bHistone Deacetylase-Related ProteinMef2-Interacting Transcription Repressor Mitr |
| Database Links | Reactome: R-HSA-2122947Reactome: R-HSA-2644606Reactome: R-HSA-2894862Reactome: R-HSA-350054 |
| Cellular Localisation | Nucleus |
| Alternative ELISA Names | Histone Deacetylase 9 ELISA kitHd9 ELISA kitHistone Deacetylase 7b ELISA kitHd7 ELISA kitHd7b ELISA kitHistone Deacetylase-Related Protein ELISA kitMef2-Interacting Transcription Repressor Mitr ELISA kitHDAC9 ELISA kitHDAC7 ELISA kitHDAC7B ELISA kitHDRP ELISA kitKIAA0744 ELISA kitMITR ELISA kit |
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Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance