Human UbcH8/UBE2L6 antibody

Product Information

Product Type

Monoclonal Antibody

Clone Number

k1H3

UniProt No.

O14933

NCBI Accession No.

NP_004214

Alternative names

Ubiquitin conjugating enzyme E2 L6, Ubiquitin/ISG15-conjugating enzyme E2 L6, E2 ubiquitin-conjugating enzyme L6, Retinoic acid-induced gene B protein, RIG-B, UbcH8, Ubiquitin carrier protein L6, Ubiquitin-protein ligase L6

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human UBE2L6 (1-152aa) purified from E. coli

Isotype

IgG2b kappa

Purification

By protein-G affinity chromatography

Applications

ELISA, WB, ICC/IF, IHC

Usage

The antibody has been tested by ELISA, Western blot, ICC/IF and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

ICC/IF analysis ofUBE2L6 in HeLa cells. The cell was stained with AUB0907 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The cell lysate of MCF7 (20ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human UBE2L6 (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

The cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UBE2L6 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: UBE2L6 Transfected 293T cell lysate

The cell lysate (20ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UBE2L6 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Jurkat cell lysate

Immunohistochemistry (IHC)

Paraffin embedded sections of human breast lobule tissue were incubated with anti-human UBE2L6 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)