IFN gamma ELISpot Assay Kit (Human)
Référence OKDB00011-1x96wells
Conditionnement : 1x96Wells
Marque : Aviva Systems Biology
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| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | Human |
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| ELISA Kit Duration | < 4 Hours |
| ELISA Kit Principle | Aviva's Human IFN gamma ELISpot Kit is based on standard sandwich ELISA methodology. A capture antibody highly specific for the analyte of interest is coated to the wells of a PVDF bottomed 96 well microtitre plate either during kit manufacture or in the laboratory. The plate is then blocked to minimise any non-antibody dependent unspecific binding and washed. Cell suspension and stimulant are added and the plate incubated allowing the specific antibodies to bind any analytes produced. Cells are then removed by washing prior to the addition of Biotinylated detection antibodies which bind to the previously captured analyte. Enzyme conjugated streptavidin is then added binding to the detection antibodies. Following incubation and washing substrate is then applied to the wells resulting in coloured spots which can be quantified using appropriate analysis software or manually using a microscope. |
| ELISA Kit Component | Reagents sufficient for 96 Wells: 1. Pre-coated 96 well PVDF bottomed plates (1x96 Wells) 2. Biotinylated detection antibody 3. Streptavidin-Alkaline Phosphatase conjugate 4. Bovine Serum Albumin 5. Ready to use BCIP/NBT substrate buffer |
| Reconstitution and Storage | 2 to 8C, Do not use past expiration date. |
| Sample Type | Cell Suspensions |
| Assay Info | Assay Methodology: Quantitative Sandwich ELISpot Immunoassay |
| Gene Symbol | IFN |
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| Gene Full Name | interferon, gamma |
| Alias Symbols | IFG, IFI, IMD69 |
| NCBI Gene Id | 3458 |
| Protein Name | interferon, gamma |
| Description of Target | This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. |
| Uniprot ID | P01579 |
| Protein Accession # | NP_000610.2 |
| Nucleotide Accession # | NM_000619.2 |
| Protein Size (# AA) | 166 |
| Molecular Weight | 19 kDa |