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> IgM, Heavy Chain (FITC)

IgM, Heavy Chain (FITC)

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Référence I1905-19B-500ug

Conditionnement : 500ug

Marque : US Biological

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I1905-19B IgM, Heavy Chain (FITC)

Clone Type
Polyclonal
Host
rat
Source
mouse
Isotype
IgG2a
Grade
Affinity Purified
Applications
FC
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications:|Sutiable for use in Flow Cytometry. Other applications not tested.||Recommended Dilutions:|Flow Cytometry: 5ug/ml|Optimal working dilutions to be determined by researcher.||Recommended Negative Controls:|Rat IgG2a Negative Control labeled with FITC||Fusion Partners: |Spleen cells from immunized LOU/c rats were fused with cells of the IR983F rat myeloma cell line.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. |Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG2a|Clone No: LO-MM-9|Host: rat|Source: mouse|Concentration: ~1mg/ml|Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol. Labeled with Fluorescein isothiocyanate (FITC) (MW 390D).|Purity: Purified by affinity chromatography.|Immunogen: Polyclonal and monoclonal mouse IgM.|Specificity: Recognizes mouse IgM mu heavy chain. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Polyclonal and monoclonal mouse IgM.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol. Labeled with Fluorescein isothiocyanate (FITC) (MW 390D).
Purity
Purified by affinity chromatography.
Specificity
Recognizes mouse IgM mu heavy chain.
References
1. Bazin, H., Cormont, F., De Clercq, L.: Rat monoclonal antibodies. II. A rapid and effective method of purification from ascitic fluid or serum. J. Immunol. Meth. 71: 9-16 (1984). 2. Bazin, H., Beckers, A., Querinjean, P.: Three classes and four (sub) classes or rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b and IgG2c. Eur. J. Immunol. 4: 44-48 (1974). 3. Gutman, G.A., Bazin, H., Rockhiin, O.V., Nezlin, R.S.: Transp. Proc. 15: 1685-1686 (1983).