IL-1B ELISA Kit (Rat)
Référence OKAG00107
Conditionnement : 1plate:12x8-WellMicrostrips
Marque : Aviva Systems Biology
Contactez votre distributeur local :
Téléphone : +1 850 650 7790
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
|---|
| Predicted Species Reactivity | Rat, Rattus norvegicus | ||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Product Format | 1 x 96-Well Plate or 12 x 8-Well Strips | ||||||||||||||||||||||
| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric 450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | 4.5 hours | ||||||||||||||||||||||
| ELISA Kit Principle | The Aviva Rat IL-1betaELISA Kit contains the components necessary for quantitative determination of natural or recombinant rIL-1betaconcentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator.The capture antibodies coated to the bottom of each well are specific for a particular epitope on theRatIL-1betacytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetricreaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration. | ||||||||||||||||||||||
| ELISA Kit Range | 47-3000 pg/ml | ||||||||||||||||||||||
| ELISA Kit Component |
| ||||||||||||||||||||||
| Reconstitution and Storage | 2°C to 8°C | ||||||||||||||||||||||
| Sample Type | Cell lysates, sera and plasma | ||||||||||||||||||||||
| Sensitivity | 47 pg/mL | ||||||||||||||||||||||
| Specificity | The Rat IL-1β ELISA is capable of recognizing both recombinant and naturally produced Rat IL-1β proteins. The antigens listed below were tested at 50 ng/ml and exhibited less than 5% cross reactivity. • Murine: IL-1β The antigens listed below were tested at 50 ng/ml and exhibited less than 1% cross reactivity. • Human: IL-1β The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference. • Human: IL-1α, IL-2, sIL-2R, IL-4, sIL-4R • Murine: IL-1α, IL-2, IL-4, IL-10 • Rat: GM-CSF, IL-1α, IL-2, IL-4, IL-10, SCF, TNFα | ||||||||||||||||||||||
| Assay Info | Quantitative Colorimentric Sandwich ELISA |
| Gene Symbol | Il1b |
|---|---|
| Gene Full Name | interleukin 1 beta |
| Alias Symbols | IL-1 beta;IL-1F2;interleukin-1 beta. |
| NCBI Gene Id | 24494 |
| Protein Name | Q63264 |
| Description of Target | Interleukin-1 beta |
| Uniprot ID | https://www.uniprot.org/uniprot/Q63264 |