IL1B ELISA Kit (Goat)

Référence OKCA00588

Conditionnement : 96Wells

Marque : Aviva Systems Biology

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityGoat
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean n=4 (%)Range (%)
Neat8782-94
1:29082-97
1:49285-100
1:89590-103
ELISA Kit PrincipleAviva Systems Biology IL1B ELISA Kit (Goat) (OKCA00588) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL1B has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL1B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IL1B captured in the well.
ELISA Kit Range3.12 pg/mL-200 pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)9085-98
EDTA Plasma (n=4)9993-108
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level IL1B were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level IL1B were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <15%
Inter-Assay CV: <15%
ELISA Kit Component
ComponentAmount
IL1B Microplate96 Wells (12 x 8 Well strips)
IL1B Lyophilized Standard2
100X Biotinylated IL1B Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.78 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
  • Reconstitution & Storage Instructions
  • Western Blotting/Immunoblotting (WB/IB) Protocol
  • Immunohistochemistry (IHC) Protocol
  • Immunocytochemistry (ICC) Protocol
  • Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
  • Blocking Peptide Competition Protocol (BPCP)
  • Immunoprecipitation (IP) Protocol
  • Antibody Array (AA) Protocol
Gene SymbolIL1B
Gene Full NameInterleukin-1 beta
Protein NameInterleukin-1 beta
Description of TargetPotent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production.
Uniprot IDP79162
Protein Accession #XP_013823154.1
Nucleotide Accession #XM_013967700.2