IPTG [367-93-1]
Référence HY-15921-5g
Conditionnement : 5g
Marque : MedChemExpress
Contactez votre distributeur local :
Téléphone : +1 850 650 7790
IPTG is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
IPTG Chemical Structure
CAS No. : 367-93-1
This product is a controlled substance and not for sale in your territory.
Based on 10 publication(s) in Google Scholar
-
IPTG purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2018 May 7;19(5). pii: E1393. [Abstract]
- Screening of thymidylate synthase (TYMS) overexpression conditions.
| Description |
IPTG is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator. |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| In Vitro |
IPTG uptake by E. coli can be independent of the action of lactose permease, since other transport pathways are also involved. At low concentration, IPTG enters cells through lactose permease, but at high concentrations (typically used for protein induction), IPTG can enter the cells independently of lactose permease[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
||||||||||||
| Masse moléculaire |
238.30 |
||||||||||||
| Formule |
C9H18O5S |
||||||||||||
| CAS No. |
367-93-1 |
||||||||||||
| Appearance |
Solid |
||||||||||||
| Color |
White to off-white |
||||||||||||
| SMILES |
O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1SC(C)C |
||||||||||||
| Livraison | Room temperature in continental US; may vary elsewhere. |
||||||||||||
| Stockage |
|
||||||||||||
| Solvant et solubilité |
In Vitro:
H2O : 100 mg/mL (419.64 mM; Need ultrasonic) DMSO : ≥ 60 mg/mL (251.78 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) *"≥" means soluble, but saturation unknown. Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles. * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
For the following dissolution methods, please prepare the working solution directly.
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration:
mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
|
||||||||||||
| Pureté et documentation |
Purity: 99.90% |
||||||||||||
| Références |
|
Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|
| DMSO / H2O | 1 mM | 4.1964 mL | 20.9820 mL | 41.9639 mL | 104.9098 mL |
| 5 mM | 0.8393 mL | 4.1964 mL | 8.3928 mL | 20.9820 mL | |
| 10 mM | 0.4196 mL | 2.0982 mL | 4.1964 mL | 10.4910 mL | |
| 15 mM | 0.2798 mL | 1.3988 mL | 2.7976 mL | 6.9940 mL | |
| 20 mM | 0.2098 mL | 1.0491 mL | 2.0982 mL | 5.2455 mL | |
| 25 mM | 0.1679 mL | 0.8393 mL | 1.6786 mL | 4.1964 mL | |
| 30 mM | 0.1399 mL | 0.6994 mL | 1.3988 mL | 3.4970 mL | |
| 40 mM | 0.1049 mL | 0.5245 mL | 1.0491 mL | 2.6227 mL | |
| 50 mM | 0.0839 mL | 0.4196 mL | 0.8393 mL | 2.0982 mL | |
| 60 mM | 0.0699 mL | 0.3497 mL | 0.6994 mL | 1.7485 mL | |
| 80 mM | 0.0525 mL | 0.2623 mL | 0.5245 mL | 1.3114 mL | |
| 100 mM | 0.0420 mL | 0.2098 mL | 0.4196 mL | 1.0491 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
IPTG Related Classifications
- Others
- Others
- Biochemical Assay Reagents