Iron Assay Kit is an assay kit designed for the detection and quantification of Iron concentrations in serum and tissue homogenates using a microplate reader. In acidic conditions and in the presence of a reducing agent, ferric ions can be separated from transferrin in serum, and reduced into ferrous ions (Fe2+). The latter then binds to bipyridine, forming pink complexes. The concentration of iron can be calculated indirectly by measuring the absorbance at 520 nm.
Research Articles on Iron
| Target | Iron |
| Reactivity | General (All species) |
| Storage | Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual. |
| Validity | The validity for this kit is 12 months. |
| Test Range | 0.29 mg/L - 10 mg/L |
| Sensitivity | 0.08 mg/L |
| Detection Method | Colorimetric |
| Assay Data | Quantitative |
| Sample Type | Serum and tissue homogenates. |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |