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> Iron Colorimetric Assay Kit

Iron Colorimetric Assay Kit

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Référence E-BC-K139-M-500

Conditionnement : 500Assays

Marque : Elabscience

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Téléphone : +1 850 650 7790

Detection principle

Under the action of acidic solution and reductant, ferric ions can be separated from transferrin in serum, and reduced into ferrous ions (Fe2+). The latter then bind to bipyridine and form pink complexes. The concentration of iron can be calculated by measuring the OD value at 520 nm indirectly.

Performance characteristics

Synonyms Fe
Sample type Serum,tissue
Sensitivity 0.08 mg/L
Detection range 0.29-10 mg/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 25 min
Precision Average inter-assay CV: 2.800%Average intra-assay CV: 1.200%
Other instruments required Micropipettor, Centrifuge, Water bath, Incubator, Vortex mixer
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 12 months

Images

The iron levels in A549 cells were significantly increased after treatment with BPQDs. (*P<0.05, **P<0.01)

F Ruan et al investigate the nanotoxicological assessment of Black Phosphorus Quantum Dots (BPQD). Iron (Fe) level in lung A549 cells were determined using iron colorimetric assay kit (E-BC-K139-M).

Dilution of sample


It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.29-10 mg/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Mouse serum

1

10% Mouse liver tissue homogenate

1

10% Rat kidney tissue homogenate

1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).


Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

  1. Bioengineered (2022) IF: 3.269
    Amentoflavone promotes ferroptosis by regulating reactive oxygen species (ROS) /5’AMP-activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR) to inhibit the malignant progression of endometrial carcinoma cells

    DOI: 10.1080/21655979.2022.2079256
    Sample: Kle Cell
  2. TOXICOLOGY (2020) IF: 4.099
    Inhibition of ferroptosis attenuates busulfan-induced oligospermia in mice

    DOI: 10.1016/j.tox.2020.152489
    Sample: Testicular Tissue
  3. BIOLOGY OF REPRODUCTION (2021) IF: 4.285
    Chronic overload of concentration-dependent iron exerts different effects on ovarian function in C57BL/6J mice

    DOI: 10.1093/biolre/ioab040
    Sample: Serum，Ovary
  4. MOLECULAR IMMUNOLOGY (2021) IF: 4.407
    Hydrogen sulfide attenuates ferroptosis and stimulates autophagy by blocking mTOR signaling in sepsis-induced acute lung injury

    DOI: 10.1016/j.molimm.2021.12.003

    PMID: 34952420

    		 Sample: Lung Tissue
  5. JOURNAL OF ETHNOPHARMACOLOGY (2023) IF: 5.195
    Verbenalin attenuates hepatic damage and mitochondrial dysfunction in alcohol-associated steatohepatitis by regulating MDMX/PPARα-mediated ferroptosis

    DOI: 10.1016/j.jep.2023.116227
    Sample: Serum,Liver Tissue,Aml-12 Cell
  6. Frontiers in Oncology (2022) IF: 6.244
    Sevoflurane Induces Ferroptosis of Glioma Cells Through Activating the ATF4-CHAC1 Pathway.

    DOI: 10.3389/fonc.2022.859621
    Sample: U251 Cell,U87 Cell
  7. Antioxidants (2022) IF: 6.313
    Hyperoxia Induces Ferroptosis and Impairs Lung Development in Neonatal Mice

    DOI: 10.3390/antiox11040641

    PMID: 35453326

    		 Sample: Lung Tissues
  8. Food & Function (2022) IF: 6.317
    Arthrospira platensis (Spirulina) fortified functional foods ameliorates iron and protein malnutrition by improving growth, modulating oxidative stress and gut microbiota in rats

    DOI: 10.1039/D2FO02226E
    Sample: Serum
  9. International Journal of Nanomedicine (2022) IF: 6.4
    A Composite Deferoxamine/Black Phosphorus Nanosheet/Gelatin Hydrogel Scaffold for Ischemic Tibial Bone Repair

    DOI: 10.2147/IJN.S351814

    PMID: 35299865

    		 Sample: Bpn-Dfo Hydrogel
  10. Nutrients (2022) IF: 6.706
    Iron Depletion in Systemic and Muscle Compartments Defines a Specific Phenotype of Severe COPD in Female and Male Patients: Implications in Exercise Tolerance

    DOI: 10.3390/nu14193929

    PMID: 36235581

    		 Sample: Muscle
  11. Antioxidants (2022) IF: 7.675
    Cathelicidin Attenuates Hyperoxia-Induced Lung Injury by Inhibiting Ferroptosis in Newborn Rats

    DOI: 10.3390/antiox11122405
    Sample: Lung Tissues
  12. ANTIOXIDANTS & REDOX SIGNALING (2021) IF: 8.401
    A novel identified circular RNA, mmu_mmu_circRNA_0000309 involves in Germacrone-mediated the improvement of diabetic nephropathy through regulating ferroptosis by targeting miR-188-3p/GPX4 signaling axis

    DOI: 10.1089/ars.2021.0063
    Sample: Kidney,Primary Mouse Podocyte Mpc5
  13. Cell Death & Disease (2022) IF: 8.469
    STAT6 inhibits ferroptosis and alleviates acute lung injury via regulating P53/SLC7A11 pathway

    DOI: 10.1038/s41419-022-04971-x

    PMID: 35668064

    		 Sample: Lung Tissue,Hbe Cell
  14. Cell Death & Disease (2022) IF: 9.685
    UHRF1-mediated ferroptosis promotes pulmonary fibrosis via epigenetic repression of GPX4 and FSP1 genes

    DOI: 10.1038/s41419-022-05515-z
    Sample: Lung Tissue
  15. JOURNAL OF CONTROLLED RELEASE (2022) IF: 9.776
    A targeted nanomodulator capable of manipulating tumor microenvironment against metastasis

    DOI: 10.1016/j.jconrel.2022.06.022

    PMID: 35716882

    		 Sample: 4 T1 Cell-Xenograft
  16. Small Methods (2021) IF: 14.188
    RNA m6A Modification Alteration by Black Phosphorus Quantum Dots Regulates Cell Ferroptosis: Implications for Nanotoxicological Assessment

    DOI: 10.1002/smtd.202001045

    PMID: 34927824

    		 Sample: Cell Sample