K2228-500 assays | Lactate Dehydrogenase (LDH) Activity Assay Kit

Description

The LDH Cytotoxicity Assay Kit is a kit that assesses cytotoxicity by measuring the activity of lactate dehydrogenase (LDH) released into the culture medium. LDH is a stable enzyme found in all cells and is released from the cell into the medium when cells are damaged or apoptosis. LDH catalyzes the conversion of lactate to pyruvate, and this catalytic process reduces NAD⁺ to NADH. The specific substrates provided in this kit can react with the resulting NADH, resulting in a product that is specifically absorbed at 490 nm. This specific absorption is directly proportional to the amount of LDH released into the culture medium Therefore, by measuring the change in absorbance, it is possible to indirectly assess the degree of cell damage or death.

Compared with the traditional ⁵¹Cr cytotoxicity test, this kit contains no radioactive substances, which is simpler and safer to use. Moreover, the detection effect of the detection method used in this kit has the same effect as that of ⁵¹Cr, and the results are reliable.

Figure 1: LDH release as measured by this kit

Quality Control

Protocol

Components and Storage

Components	K2228-200T	K2228-1000T
Substrate Mix	1 vial	5 x 1 vial
Assay Buffer	1 mL	5 x 1 mL
Lysis Buffer (10X)	2.5 mL	12 mL
Stop Solution	12 mL	60 mL
LDH Positive Control	6 μL	30 μL
Store the kit at -20°C, stable for 1 year. Substrate Mix should be stored away from light.

Lactate Dehydrogenase (LDH) Activity Assay Kit

Référence K2228-500 assays

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Description

Quality Control

Components and Storage

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