Macrophage (L1 Protein, Calprotectin, Cystic Fibrosis Antigen) (PE)

Référence M1202-76-PE-100ul

Conditionnement : 100ul

Marque : US Biological

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M1202-76-PE Macrophage (L1 Protein, Calprotectin, Cystic Fibrosis Antigen) (PE)

Clone Type
Polyclonal
Host
mouse
Source
human
Swiss Prot
P06702
Isotype
IgG1
Grade
Affinity Purified
Applications
FC IHC
Crossreactivity
Bo Ca Eq Fe Gt Gp Hu Mk Po Rb Rt
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

The L1 or Calprotectin molecule is an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. The antigen is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia.||Applications:|Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.||Recommended Dilutions:|Flow Cytometry: 10ul labels 1x10e6 cells in 100ul. Membrane permeabilization is required for this application.|Immunohistochemistry (Frozen and Paraffin): Prior to staining paraffin sections, product requires protein digestion pre-treatment e.g. trypsin, 0.1% for 10 minutes or antigen retrieval using heat treatment. Frozen sections do not require pretreatment.|Optimal working dilutions to be determined by researcher.||Recommended Positive Control:|Human spleen||Recommended Negative Control: |I1904-78R: IgG1: Murine Negative Control||Recommended Secondary Antibodies:|I1904-65W: IgG, F(ab’)2 (HRP) Pab Rb x Mo|I1904-66C: IgG, F(ab’)2 X-adsorbed (HRP) Pab Rb x Mo|I1904-66A: IgG, F(ab’)2 (FITC) Pab Rb x Mo||Hybridoma: |NS1 myeloma cells with spleen cells from Balb/c mice.||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. ||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 3H2617 (MAC387)|Host: mouse|Source: human|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).|Purity: Purified by Protein G affinity chromatography.|Immunogen: Human monocytes|Specificity: Recognizes human L1 or Calprotectin molecule. Species Crossreactivity: Rhesus and Cynomolgus monkey, baboon, feline, canine, guinea pig, equine, rabbit, rat, goat, porcine, bovine and Fallow deer.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Human monocytes
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human L1 or Calprotectin molecule. Species Crossreactivity: Rhesus and Cynomolgus monkey, baboon, feline, canine, guinea pig, equine, rabbit, rat, goat, porcine, bovine and Fallow deer.
References
Grosche, A. et al., (2013) Am. J. Veterinary Research 74; 1281-1290. 1. Brandtzaeg, P., et al., J. Clin. Path. 41: 963-970 (1988). 2. Brandtzaeg, P., et al., Histopathol. 21: 191-196 (1992). 3. Flavell, D.J., Jones, D.B. and Wright, D.H., J. Histochem. Cytochem. 35: 1217-1226 (1987). 4. Gutierrez, M., et al., Vet. Immunol. Immunopathol. 71: 321-334 (1999). 5. Ramsay, A.D., et al., Journal of Pathology 164: 321-328 (1991). 6. Christgau, M., et al., Journal of Histochemistry & Cytochemistry 46(12): 1443-1454 (1998). 7. Perez, J., et al., J. Comp. Path. 121: 385-397 (1999). 8. Obert, L., et al., J. Virol. 76(12): 6311-6322 (2002). 9. Malik, N., et al., Circulation 98: 1657-1665 (1998). 10. Bagavant, H., et al., Am. J. Pathol. 160: 141-149 (2002).