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> MAP28 ELISA Kit (Goat)

MAP28 ELISA Kit (Goat)

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Référence OKWB00141

Conditionnement : 96Wells

Marque : Aviva Systems Biology

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Datasheets/ManualsPrintable datasheet for MAP28 ELISA Kit (Goat) (OKWB00141)
Product Info
Predicted Species ReactivityCapra hircus|Goat
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit Linearity
Sample Type1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)90-105%85-105%98-102%88-101%
EDTA Plasma (n=5)85-101%85-101%89-106%86-97%
Heparin Plasma (n=5)85-98%82-95%87-100%86-98%
ELISA Kit PrincipleAviva Systems Biology Map28 ELISA Kit (Goat) (OKWB00141) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Map28 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Map28 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Map28 captured in the well.
ELISA Kit Range0.156-10ng/ml
ELISA Kit Recovery
Sample TypeRangeMean
Serum (n=5)86-9994
EDTA Plasma (n=5)86-10395
Heparin Plasma (n=5)88-10496
ELISA Kit ReproducibilityMean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%
ELISA Kit Component
ComponentAmount
Map28 Microplate96 Wells (12 x 8 Well strips)
Map28 Lyophilized Standard2 x 10 ng
100X Map28 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Sensitivity0.094 ng/mL
Assay InfoAssay Type: Quantitative Sandwich ELISA
Gene SymbolMAP28
Gene Full NameMAP28 protein
Alias SymbolsMAP28 protein.
NCBI Gene Id100861192
Protein NameMAP28 protein
Uniprot IDQ9XSQ8
Protein Accession #NP_001272475
Nucleotide Accession #NM_001285546