Methylene blue trihydrate
Référence M13990-5g
Conditionnement : 5g
Marque : AbMole Bioscience
C.I. Basic Blue 9 trihydrate
Quality Control & Documentation
Biological Activity
Methylene blue trihydrate (C.I. Basic Blue 9 trihydrate) is a guanylyl cyclase (sGC), monoamine oxidase A (MAO-A) and NO synthase (NOS) inhibitor. Methylene blue trihydrate is a vasopressor and is often used as a dye in several medical procedures. Methylene blue trihydrate has antinociception, antimalarial, antidepressant and anxiolytic activity effects. Methylene Blue trihydrate has the potential for methemoglobinemias, neurodegenerative disorders and ifosfamide-induced encephalopathytreatment.
Chemical Information
Molecular Weight | 373.9 |
Formula | C16H20ClN3OS |
CAS Number | 7220-79-3 |
Form | Solid |
Solubility (25°C) | DMSO 90 mg/mL |
Storage | Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
Conversion of different model animals based on BSA (PMID: 27057123)
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
References
[1] Peijun Xu, et al. Binding modes and interaction mechanism between different base pairs and methylene blue trihydrate: a quantum mechanics study
[2] Scott S Auerbach, et al. Toxicity and carcinogenicity studies of methylene blue trihydrate in F344N rats and B6C3F1 mice
[3] National Toxicology Program. Toxicology and carcinogenesis studies of methylene blue trihydrate (Cas No. 7220-79-3) in F344/N rats and B6C3F1 mice (gavage studies)
[4] Felix Gut, et al. Cation exchange resins as pharmaceutical carriers for methylene blue: binding and release
[5] Norman F Gaudette, et al. Determination of methylene blue and leucomethylene blue in male and female Fischer 344 rat urine and B6C3F1 mouse urine