MMP9 ELISA Kit (Dog)

Référence OKCD02730

Conditionnement : 96Wells

Marque : Aviva Systems Biology

Demander plus d'informations

Contactez votre distributeur local :


Téléphone : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for MMP9 ELISA Kit (Dog) (OKCD02730)
Product Info
Predicted Species ReactivityCanis lupus|Dog
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)94-102%80-102%94-101%84-97%
heparin plasma(n=5)86-97%87-94%80-94%80-91%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 9 (MMP9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 9 (MMP9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 9 (MMP9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Matrix Metalloproteinase 9 (MMP9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range3.12-200ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)90-9894
Heparin Plasma(n=5)82-10190
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 9 (MMP9) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 9 (MMP9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-MMP9 Microplate96 Wells (12 x 8 Well strips)
MMP9 Lyophilized Standard2
100X Biotinylated MMP9 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity< 1.14 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Macrophage Inflammatory Protein 1 Alpha (MIP1a). No significant cross-reactivity or interference between Macrophage Inflammatory Protein 1 Alpha (MIP1a) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
  • Reconstitution & Storage Instructions
  • Western Blotting/Immunoblotting (WB/IB) Protocol
  • Immunohistochemistry (IHC) Protocol
  • Immunocytochemistry (ICC) Protocol
  • Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
  • Blocking Peptide Competition Protocol (BPCP)
  • Immunoprecipitation (IP) Protocol
  • Antibody Array (AA) Protocol
Gene SymbolMMP9
Alias Symbols92 kDa gelatinase;92 kDa type IV collagenase;Gelatinase B.
Protein NameMatrix metalloproteinase-9
Description of TargetCould play a role in bone osteoclastic resorption. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (By similarity).
Uniprot IDO18733
Protein Accession #NP_001003219.1
Nucleotide Accession #NM_001003219.2
Protein Size (# AA)704