Mouse Anti-Chicken CD8α-APC
Référence 8220-11
Conditionnement : 0.1mg
Marque : Southern Biotech
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| Clone | CT-8 |
|---|---|
| Isotype | Mouse (BALB/c) IgG1κ |
| Isotype Control | Mouse IgG1-APC (15H6) |
| Specificity | Chicken/Turkey/Guinea Fowl/Pigeon CD8α |
| Description | In the chicken, the CD8 molecule is present in two forms - (i) a homodimer of two α chains and (ii) a heterodimer of an α chain and a β chain. While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8α- and CD8β-chains, a relatively large proportion of the CD8+ TCRγδ cells in the spleens of embryos and young chicks express only the α-chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved but there is a population of TCRγδ CD8αβ cells in the chicken that is not found in rodents. Chicken CD8 is expressed on approximately 80% of thymocytes, 15% of blood mononuclear cells, and 50% of spleen cells but less than 1% of cells in the bursa and bone marrow. The monoclonal antibody CT-8 recognizes the CD8α chain and has been shown to react to a polymorphic determinant in turkey. |
| Immunogen | Chicken thymocytes and Ig-negative blood leukocytes |
| Conjugate | APC (Allophycocyanin) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer |
| Clonality | Monoclonal |
| Concentration | 0.1 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light; Do not freeze |
| Applications | Flow Cytometry – Quality tested 1,9-16,18 Immunohistochemistry-Frozen Sections – Reported in literature 2-5 Immunohistochemistry-Paraffin Sections – Reported in literature 6-8 Immunocytochemistry - Reported in literature 17 Immunoprecipitation – Reported in literature 1,17 Blocking – Reported in literature 1 |
| RRID Number | AB_2796442 |
| Gene ID | 403158 (Chicken) 100126556 (Turkey) 102085944 (Pigeon) |
| Gene ID Symbol | CD8A (Chicken) LOC100126556 (Turkey) CD8A (Pigeon) |
| Gene ID Aliases | CD8 CD8A; CD8-alpha |
Documentation
Technical Bulletin Safety Datasheet
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- 2. Tanimura N, Sharma JM. Appearance of T cells in the bursa of Fabricius and cecal tonsils during the acute phase of infectious bursal disease virus infection in chickens. Avian Dis. 1997;41:638-45. (IHC-FS)
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