Mouse Anti-Human CD57-AF647
Référence 9665-31
Conditionnement : 100tests
Marque : Southern Biotech
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Téléphone : +1 850 650 7790
| Clone | NK-1 |
|---|---|
| Isotype | Mouse IgMκ |
| Isotype Control | 0101-31 |
| Specificity | Human CD57 |
| Alternative Names | HNK-1, Leu-7, B3GAT1 |
| Description | CD57 represents a 110 kDa oligosaccharide antigenic determinant present on a variety of polypeptides, lipids, and chondroitan sulfate proteoglycans. It is expressed on 7-35% of normal peripheral blood lymphocytes, including subsets of NK cells and CD8+ T lymphocytes. The carbohydrate is expressed on various cell adhesion molecules in the nervous system and is suggested to play a role in cell–cell and cell–substrate interactions. |
| Immunogen | Membrane extract of the human lymphoblastoid cell line HSB-2 |
| Conjugate | AF647 (Alexa Fluor® 647) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
| Clonality | Monoclonal |
| Concentration | Lot specific |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light |
| Trademark Information | Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries |
| Applications | Flow Cytometry – Quality tested 1-4 Immunohistochemistry-Frozen Sections – Reported in literature 5 Immunohistochemistry-Paraffin Sections – Reported in literature 6-8 Immunocytochemistry – Reported in literature 2,3 Immunoprecipitation – Reported in literature 8 Western Blot – Reported in literature 2,3,8,9 Purification – Reported in literature 9 Blocking – Reported in literature 2,8 Adhesion – Reported in literature 2 |
| RRID Number | AB_2797059 |
| Gene ID | 27087 (Human) |
| Gene ID Symbol | B3GAT1 (Human) |
| Gene ID Aliases | CD57; GLCATP; GLCUATP; HNK1; LEU7; NK-1; NK1 |
| UniProt ID | Q9P2W7 (Human |
| UniProt Name | B3GA1_HUMAN (Human) |
Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup
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- 1. Abo T, Balch CM. A differentiation antigen of human NK and K cells identified by a monoclonal antibody (HNK-1). J Immunol. 1981;127:1024-9. (Immunogen, FC)
- 2. Miura R, Ethell IM, Yamaguchi Y. Carbohydrate-protein interactions between HNK-1-reactive sulfoglucuronyl glycolipids and the proteoglycan lectin domain mediate neuronal cell adhesion and neurite outgrowth. J Neurochem. 2001;76:413-24. (FC, ICC, WB, Block, Adhesion)
- 3. Yanagisawa M, Taga T, Nakamura K, Ariga T, Yu RK. Characterization of glycoconjugate antigens in mouse embryonic neural precursor cells. J Neurochem. 2005;95:1311-20. (FC, ICC, WB)
- 4. Bentebibel S, Schmitt N, Banchereau J, Ueno H. Human tonsil B-cell lymphoma 6 (BCL6)-expressing CD4+ T-cell subset specialized for B-cell help outside germinal centers. Proc Natl Acad Sci USA. 2011;108:E488-97. (FC)
- 5. Liu AY, Roudier MP, True LD. Heterogeneity in primary and metastatic prostate cancer as defined by cell surface CD profile. Am J Pathol. 2004;165:1543-56. (IHC-FS)
- 6. Jensen HK, Donskov F, Marcussen N, Nordsmark M, Lundbeck F, von der Maase H. Presence of intratumoral neutrophils is an independent prognostic factor in localized renal cell carcinoma. J Clin Oncol. 2009;27:4709-17. (IHC-PS)
- 7. Zou Y, Chen T, Han M, Wang H, Yan W, Song G, et al. Increased killing of liver NK cells by Fas/Fas ligand and NKG2D/NKG2D ligand contributes to hepatocyte necrosis in virus-induced liver failure. J Immunol. 2010;184:466-75. (IHC-PS)
- 8. Suzuki-Anekoji M, Suzuki M, Kobayashi T, Sato Y, Nakayama J, Suzuki A, et al. HNK-1 glycan functions as a tumor suppressor for astrocytic tumor. J Biol Chem. 2011;286:32824-33. (IHC-PS, IP, WB, Block)
- 9. Yoshihara Y, Oka S, Watanabe Y, Mori K. Developmentally and spatially regulated expression of HNK-1 carbohydrate antigen on a novel phosphatidylinositol-anchored glycoprotein in rat brain. J Cell Biol. 1991;115:731-44. (WB, Purification)