Images
Western Blot
Application

Western Blot

Western blot analysis of nonreduced (-) and reduced (+) mouse immunoglobulins (20 ng/lane) with Mouse IgG monoclonal antibody, clone RM104 (Biotin) (Cat # MAB14934) at 0.2 ug/mL working concentration. This antibody reacts to nonreduced mouse IgG1, IgG2a, IgG2b, and IgG3. It showed no cross reactivity with IgM, IgA, or IgE.

Enzyme-linked Immunoabsorbent Assay
Application

Enzyme-linked Immunoabsorbent Assay

ELISA analysis of Mouse IgG monoclonal antibody, clone RM104 (Cat # MAB12765) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the Fc region of mouse IgG1, IgG2a, IgG2b, and IgG3; no cross reactivity with IgM, IgA, IgE, human or rat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • Specifications

    Product Description

    Rabbit recombinant monoclonal antibody raised against mouse IgG.Recombinant Antibody,Recombinant Antibodies,Recombinant Monoclonal Antibody,RecomAb,Recombinant Ab,Recombinant Monoclonal Antibodies,Recombinant Abs

    Antibody Species

    Rabbit

    Immunogen

    Original antibody is raised against mouse IgG.

    Sequence

    N/A

    Reactivity

    Mouse

    Form

    Liquid

    Conjugation

    Biotin

    Purification

    Protein A purification

    Isotype

    IgG

    Recommend Usage

    ELISA (0.005-0.2 ug/mL)
    Flow Cytometry
    Immunocytochemistry (0.5-2 ug/mL)
    Immunohistochemistry (0.5-2 ug/mL)
    Immunoprecipitation
    Western Blotting (0.1-0.5 ug/mL)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)

    Storage Instruction

    Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot

    Western blot analysis of nonreduced (-) and reduced (+) mouse immunoglobulins (20 ng/lane) with Mouse IgG monoclonal antibody, clone RM104 (Biotin) (Cat # MAB14934) at 0.2 ug/mL working concentration. This antibody reacts to nonreduced mouse IgG1, IgG2a, IgG2b, and IgG3. It showed no cross reactivity with IgM, IgA, or IgE.

    Immunohistochemistry

    Immunocytochemistry

    Immunoprecipitation

    Enzyme-linked Immunoabsorbent Assay

    ELISA analysis of Mouse IgG monoclonal antibody, clone RM104 (Cat # MAB12765) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the Fc region of mouse IgG1, IgG2a, IgG2b, and IgG3; no cross reactivity with IgM, IgA, IgE, human or rat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

    Flow Cytometry

  • Gene Info — Ighg1

    Entrez GeneID

    16017

    Protein Accession#

    P01868

    Gene Name

    Ighg1

    Gene Alias

    IgG1, Igh-4, VH7183

    Gene Description

    immunoglobulin heavy constant gamma 1 (G1m marker)

    Gene Ontology

    Hyperlink

    Other Designations

    -