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> Mouse IgG1 antibody (Alkaline Phosphatase)

Mouse IgG1 antibody (Alkaline Phosphatase)

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Référence orb347574-1mg

Conditionnement : 1mg

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    Mouse IgG1 antibody (Alkaline Phosphatase)

    Catalog Number: orb347574

    Catalog Numberorb347574
    CategoryAntibodies
    DescriptionMouse IgG1 antibody (Alkaline Phosphatase)
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsDOT, ELISA, IHC, WB
    ReactivityMouse
    IsotypeIgG
    ImmunogenMouse IgG1 heavy chain
    Concentration1.0 mg/mL
    Dilution rangeELISA: 1:5,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
    Form/AppearanceLiquid (sterile filtered)
    PurityIgG1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Rabbit Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
    ConjugationAP
    StorageStore vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
    Buffer/Preservatives0.01% (w/v) Sodium Azide
    Alternative namesRabbit Anti-Mouse IgG1 (Gamma 1 chain) Antibody al
    NoteFor research use only
    Application notesAnti-Mouse IgG1 Alkaline Phosphatase Antibody has been tested by ELISA, dot blot, and western blot and is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1 antibody based assays.
    Expiration Date12 months from date of receipt.
    Mouse IgG1 antibody (Alkaline Phosphatase)

    Dot Blot of Mouse IgG1 Secondary Antibody Alkaline Phosphatase Conjugated. Antigen: Mouse IgG1. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.4 ng Lane 5 - 2.5 ng. Primary antibody: none. Secondary antibody: Mouse IgG1 Secondary Antibody Alkaline Phosphatase Conjugated at 1:1000 for one hour at RT. Block: orb348637 for 60 min at RT. Reaction visualized using alkaline phosphatase substrate for 30 seconds at RT.

    Mouse IgG1 antibody (Alkaline Phosphatase)

    Western Blot of Mouse IgG1 Secondary Antibody Alkaline Phosphatase Conjugated. Lane 1: Mouse IgG1. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Mouse IgG1 Secondary Antibody Alkaline Phosphatase Conjugated at 1:1000 o/n at 4°C. Block: orb348637 for 30 min at RT. Reaction visualized using alkaline phosphatase substrate for 30 seconds. Predicted/Observed size: 55 kDa for Mouse IgG1.