Images
Western Blot
Application

Western Blot

Western blot analysis of Lane 1: nonreduced (-) and Lane 2: reduced (+) mouse IgG2b (20 ng/lane) with Mouse IgG2b monoclonal antibody, clone RM108 (Cat # MAB12769) under 0.2 ug/mL working concentration. This antibody reacts to nonreduced IgG2b (~150 kDa) much stronger than the reduced gamma 2b form (~50 kDa).

Enzyme-linked Immunoabsorbent Assay
Application

Enzyme-linked Immunoabsorbent Assay

ELISA analysis of Mouse IgG2b monoclonal antibody, clone RM108 (Cat # MAB12769) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the Fc region of mouse IgG2b; no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • Specifications

    Product Description

    Rabbit recombinant monoclonal antibody raised against mouse IgG2b.Recombinant Antibody,Recombinant Antibodies,Recombinant Monoclonal Antibody,RecomAb,Recombinant Ab,Recombinant Monoclonal Antibodies,Recombinant Abs

    Antibody Species

    Rabbit

    Immunogen

    Original antibody is raised against mouse IgG2b.

    Sequence

    N/A

    Reactivity

    Mouse

    Specificity

    This antibody reacts to the Fc region of mouse IgG2b. No cross reactivity with mouse IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.

    Form

    Liquid

    Purification

    Protein A affinity purification

    Isotype

    IgG

    Recommend Usage

    ELISA (0.005 - 0.2 ug/mL)
    Flow Cytometry
    Immunocytochemistry (0.5 - 2 ug/mL)
    Immunohistochemistry (0.5 - 2 ug/mL)
    Immunoprecipitation
    Western Blot (0.1 - 0.5 ug/mL)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)

    Storage Instruction

    Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot

    Western blot analysis of Lane 1: nonreduced (-) and Lane 2: reduced (+) mouse IgG2b (20 ng/lane) with Mouse IgG2b monoclonal antibody, clone RM108 (Cat # MAB12769) under 0.2 ug/mL working concentration. This antibody reacts to nonreduced IgG2b (~150 kDa) much stronger than the reduced gamma 2b form (~50 kDa).

    Immunohistochemistry

    Immunocytochemistry

    Immunoprecipitation

    Enzyme-linked Immunoabsorbent Assay

    ELISA analysis of Mouse IgG2b monoclonal antibody, clone RM108 (Cat # MAB12769) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, and 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the Fc region of mouse IgG2b; no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

    Flow Cytometry

  • Gene Info — Igh-3

    Entrez GeneID

    16016

    Protein Accession#

    P01867

    Gene Name

    Igh-3

    Gene Alias

    IGHG2B, IgG2b, gamma2b

    Gene Description

    immunoglobulin heavy chain 3 (serum IgG2b)

    Gene Ontology

    Hyperlink

    Other Designations

    -