Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.
 
> GFP Tag Antibody

GFP Tag Antibody

Imprimer Imprimer
Identifiez-vous

Référence AM1009a

Conditionnement : 200ul

Marque : Abcepta

Demander plus d'informations

Contactez votre distributeur local :


Téléphone : +1 850 650 7790

 
  • IF - GFP Tag Antibody AM1009A
    Immunofluorescent analysis of GFP using either natural fluorescence (green) or an GFP antibody (red) in Hela(human cervical epithelial adenocarcinoma cell line) cells transfected with GFP recombinant protein. Formalin-fixed cells were permeabilized with 0. 1% Triton X-100 for 10 minutes at room temperature and blocked with 3% BSA for 30 minutes at room temperature. Cells were probed with an GFP monoclonal antibody (Product # AM1009a) at a dilution of 1:25 for 1 hour at 37C, and incubated with DyLight 555 goat anti-mouse IgG secondary antibody (Product # 1511348) at a dilution of 1:200 for 60 minutes at 37℃. The nuclear counter stain is DAPI (blue).
  • WB - GFP Tag Antibody AM1009A
    Anti-GFP Tag Antibody at dilution + GFP protein whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession C5MKY7
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG1
Clone/Animal Names 168AT1211
Calculated MW 27 k KDa
Additional Information
Target/Specificity Purified His-tagged GFP protein was used to produced this monoclonal antibody.
Dilution IF~~1:25
WB~~1:20000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGFP Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information