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Mouse Monoclonal Anti-VLDL-Receptor (Very Low Density Lipoprotein Receptor) [Clone VLDLR/1337]
Référence NB-36-00851-P0
Conditionnement : 20ug
Marque : Neo Biotech
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Printable Datasheet
VLDL-Receptor (Very Low Density Lipoprotein Receptor) Antibody [VLDLR/1337]
Catalog Number Formulation Size Price
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Pancreas stained with VLDL-Receptor Mouse Monoclonal Antibody (VLDLR/1337).
SDS-PAGE Analysis of Purified VLDL-Receptor Mouse Monoclonal Antibody (VLDLR/1337). Confirmation of Purity and Integrity of Antibody.
Applications & Dilutions
Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Summary
VLDLR (very low density lipoprotein receptor) is a member of the LDL receptor gene family, which includes LDL receptor, LRP, megalin, VLDLR and ApoER2. The LDL receptor family is characterized by a cluster of cysteine-rich class A repeats, epidermal growth factor (EGF)-like repeats, YWTD repeats and an O-linked sugar domain. VLDLR associates with RAP (receptor associated protein) during receptor folding, and RAP facilitates the secretion of the extracellular region of VLDLR. VLDLR is thought to mediate the interaction of extracellular Reelin and cytosolic mDab1 (mammalian disabled protein), which activates a tyrosine kinase. This pathway regulates the migration of neurons along the radial glial fiber network during brain development.
Product Properties & Targets
Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human, Rat
Research Areas
Cardiovascular, Developmental Biology
Isotype / Light Chain
IgG1 / Lambda
Cellular Localization
Cell membrane, Clathrin-coated pit, Membrane
Gene Name
VLDLR
Positive Control
Cerebellum, Heart or Skeletal Muscle., HeLa or U-251 cells. Pancreas
Immunogen
Recombinant human VLDLR fragment from c-terminal
Alternate Names
Very low-density lipoprotein receptor, Very low-density lipoprotein receptor; VLDL receptor; VLDL-R; VLDLR
Functions
- Multifunctional cell surface receptor that binds VLDL and transports it into cells by endocytosis and therefore plays an important role in energy metabolism. Binds also to a wide range of other molecules including Reelin/RELN or apolipoprotein E/APOE-containing ligands as well as clusterin/CLU (PubMed:24381170, PubMed:30873003). In the off-state of the pathway, forms homooligomers or heterooligomers with LRP8 (PubMed:30873003). Upon binding to ligands, homooligomers are rearranged to higher order receptor clusters that transmit the extracellular RELN signal to intracellular signaling processes by binding to DAB1 (PubMed:30873003). This interaction results in phosphorylation of DAB1 leading to the ultimate cell responses required for the correct positioning of newly generated neurons. Later, mediates a stop signal for migrating neurons, preventing them from entering the marginal zone (By similarity).
- (Microbial infection) Acts as a receptor for Semliki Forest virus.
Key References
- Mikhailenko, I., et al. 1999. J. Cell Sci. 112: 3269-3281.
PubMed Links
- https://pubmed.ncbi.nlm.nih.gov/8128315/
- https://pubmed.ncbi.nlm.nih.gov/8069294/
- https://pubmed.ncbi.nlm.nih.gov/8294473/
- https://pubmed.ncbi.nlm.nih.gov/8020981/
- https://pubmed.ncbi.nlm.nih.gov/15164053/
- https://pubmed.ncbi.nlm.nih.gov/15489334/
- https://pubmed.ncbi.nlm.nih.gov/12857919/
- https://pubmed.ncbi.nlm.nih.gov/17330141/
- https://pubmed.ncbi.nlm.nih.gov/18039658/
- https://pubmed.ncbi.nlm.nih.gov/20427281/
- https://pubmed.ncbi.nlm.nih.gov/23701949/
- https://pubmed.ncbi.nlm.nih.gov/24381170/
- https://pubmed.ncbi.nlm.nih.gov/30873003/
- https://pubmed.ncbi.nlm.nih.gov/34929721/
- https://pubmed.ncbi.nlm.nih.gov/15064754/
- https://pubmed.ncbi.nlm.nih.gov/10391209/
- https://pubmed.ncbi.nlm.nih.gov/16080122/
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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