Mouse PCSK9 protein (Recombinant)

Conjugation:	Unconjugated
Formulation:	Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose.
Storage Instruction:	Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA
Endotoxin:	Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA
Immunoreactivity:	The activity was tested by the ability of immobilized recombinant Mouse PCSK9 to bind recombinant human LDLR within a linear range of 10-100ng/ml in ELISA. NA

Gene Symbol:	Pcsk9
Gene ID:	100102
Uniprot ID:	PCSK9_MOUSE
Immunogen Region:	ECD
Immunogen:	Optimized DNA sequence encoding extracellular domain of Mouse Proprotein convertase subtilisin/kexin type 9 (PCSK9) (GLN35-GLN694) including an N-terminal His tag was expressed in HEK293 cells. NA

Tissue Specificity	Hepatocytes, kidney mesenchymal cells, intestinal ileum, colon epithelia and embryonic brain telencephalon neurons.
Post Translational Modifications	Cleavage by furin and PCSK5 generates a truncated inactive protein that is unable to induce LDLR degradation. Undergoes autocatalytic cleavage in the endoplasmic reticulum to release the propeptide from the N-terminus and the cleavage of the propeptide is strictly required for its maturation and activation. The cleaved propeptide however remains associated with the catalytic domain through non-covalent interactions, preventing potential substrates from accessing its active site. As a result, it is secreted from cells as a propeptide-containing, enzymatically inactive protein. Phosphorylation protects the propeptide against proteolysis.
Function	Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments. Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation. Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway. Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways.
Protein Name	Proprotein Convertase Subtilisin/Kexin Type 9 Neural Apoptosis-Regulated Convertase 1 Narc-1 Proprotein Convertase 9 Pc9 Subtilisin/Kexin-Like Protease Pc9
Database Links	Reactome: R-MMU-381426 Reactome: -MMU-8866427 Reactome: -MMU-8957275 Reactome: -MMU-8964038
Cellular Localisation	Cytoplasm Secreted Endosome Lysosome Cell Surface Endoplasmic Reticulum Golgi Apparatus Autocatalytic Cleavage Is Required To Transport It From The Endoplasmic Reticulum To The Golgi Apparatus And For The Secretion Of The Mature Protein Localizes To The Endoplasmic Reticulum In The Absence Of Ldlr And Co-Localizes To The Cell Surface And To The Endosomes/Lysosomes In The Presence Of Ldlr The Sorting To The Cell Surface And Endosomes Is Required In Order To Fully Promote Ldlr Degradation
Alternative Protein Names	Proprotein Convertase Subtilisin/Kexin Type 9 protein Neural Apoptosis-Regulated Convertase 1 protein Narc-1 protein Proprotein Convertase 9 protein Pc9 protein Subtilisin/Kexin-Like Protease Pc9 protein Pcsk9 protein Narc1 protein

Information sourced from Uniprot.org

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